Research on the Optimization of Ultrasonic-Assisted Ethanol Extraction of Total Flavonoids from Mentha Haplocalyx Using Response Surface Methodology
Based on response surface analysis,it optimizes the conditions for ultrasonic extraction of total flavonoids from Mentha haplocalyx,and compares the differences in total flavonoid content between dried and fresh mint,fresh mint stems,and fresh mint leaves.The experiment adopts response surface analysis method and obtains the optimal extraction parameters through a series of single factor and response surface design experiments.The material liquid ratio is 1∶53(g/mL),the ethanol concentration is 30%,the ex-traction temperature is 50℃,and the extraction time is 69 minutes.Under the aforementioned extraction conditions,the total flavonoid yields in dried mint,fresh mint leaves,and fresh mint stems are determined to be 3.4%,9.64%and 9.75%,respectively,using UV spectrophotometry.Spiked recovery tests are con-ducted on the samples,resulting in recovery rates of 99.5%,100.2%and 101.9%.Mastering the signifi-cant difference in total flavonoid content between fresh mint and dried mint is of great significance for the pharmaceutical and food additive industries.
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