首页|核酸提取装置的移液精度优化设计研究

核酸提取装置的移液精度优化设计研究

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为了提高核酸提取装置的移液精度和稳定性,以基于空气置换原理、活塞驱动方式的移液模块作为研究对象,提出了一种基于正弦函数的S型加减速曲线算法,并将其与匀加减速、抛物线型加减速曲线算法进行了仿真对比和实验验证.结果表明:采用基于正弦函数的S型加减速曲线算法的核酸提取装置,在移液量为10µL、20µL、50µL、100µL和200µL的不同工作条件下,移液精度均取得了显著的提升,同时实验数据的CV值满足JJG 646-2006移液器检定规程.
Optimization Design of Pipetting Accuracy for Nucleic Acid Extraction Device
In order to improve the pipetting accuracy and stability of the nucleic acid extraction device ,an S-type accelera-tion and deceleration curve algorithm based on sinusoidal function was proposed by taking the pipetting module based on the principle of air displacement and piston drive as the research object ,and it was compared with the uniform acceleration and deceleration and parabolic acceleration and deceleration curve algorithms and verified by experiments. The results showed that the nucleic acid extraction device based on the S-type acceleration and deceleration curve algorithm based on the sinu-soidal function achieved a significant improvement in the pipetting accuracy under different working conditions of 10 μL , 20 μL ,50 μL ,100 μL and 200 μL ,and the CV value of the experimental data met the JJG 646-2006 pipette verification regulations.

stepper motormicro liquid handling technologyS-type acceleration and deceleration curve algorithmpipetting accuracy

吕阳、宫平、王哲、于源华、端木路阳

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长春理工大学 生命科学技术学院,长春 130022

步进电机 微量液体处理技术 S型加减速曲线算法 移液精度

吉林省科技厅项目

20220505013ZP

2024

长春理工大学学报(自然科学版)
长春理工大学

长春理工大学学报(自然科学版)

CSTPCD
影响因子:0.432
ISSN:1672-9870
年,卷(期):2024.47(2)
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