In order to further study the related functional genes for Pseudomonas aeruginosa in response to anaerobic stress environment and maintaining long-term survival through pyruvate fermentation,homologous recombination sucrose reverse screening technology was used to construct universal stress protein uspK gene knockout mutant strain ΔPA3309,and detected the phenotype and genotype differences between the wild type strain PAO1 and the mutant strain under anaerobic pyruvate fermentation condition. The phenotype differences of bacteria survival curves and NADH/NADPH amount showed that the mutant strain was still in a high energy state under anaerobic stress conditions than PAO1. In additional,the genotype differences of transcriptomic data showed that energy metabolism genes and signaling related genes of PAO1 strain were significantly lower than mutant strain. Compared with mutant strain,the TetR and LysR family transcription regulator were down regulated nearly 220,and the SDR family oxidation reductase was down regulated more than 215 in PAO1,indicating that PAO1 may regulate TetR and LysR transcription factors through the UspK protein,further down regulating energy metabolism proteins such as SDR family oxidation reductase under anaerobic stress pyruvate fermentation conditions.
维普
万方数据