Label-free Electrochemical Aptasensor Based on cMWCNTs/Cys for Quantitative Detection of Myoglobin
Aptamers are used as molecular recognition and binding sensitive elements,and the functionalized electrode as the transducer element,a label-free electrochemical aptamer-based sensor was constructed and quantitative detected for serum myoglobin samples. In this study,the amino,carboxyl and sulfhydryl group of L-Cys and carboxylated multi-wall carbon nanotubes (cMWCNTs) were covalently connected to the surface of the 5′ modified amino aptamer and the gold disk base electrode,and the Apt/cMWCNTs/Cys modified functional electrode was obtained and electrochemical properties character-ization. The results showed that the differential pulse peak current value of the sensing system has a good linear relationship with the logarithm of Mb concentration from 0.01～10 ng/mL,the correlation coefficient was 0.993. The cMWCNTs/Cys label-free electrochemical aptasensor has good specificity for serum myoglobin quantitative detection. The limit of detection (LOD) was 0.016 pg/mL,the relative standard deviation (RSD) was 2.09％～3.10％,and the recovery rate was 94.26％～101.30％.
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