NiFe2O4磁性纳米粒子制备及其吸附富组氨酸蛋白的研究
Preparation of NiFe2O4 Magnetic Nanoparticles for Affinity Adsorption of Histidine-Rich Proteins
佟彤 1刘思齐 2单海霞 3李海彦1
作者信息
- 1. 长春理工大学 生命科学技术学院,长春 130022
- 2. 长春理工大学 物理学院,长春 130022
- 3. 吉林大学出版社 长春 130021
- 折叠
摘要
引入具有生物相容性、亲水性好的高分子材料聚乙二醇,通过水热法一步合成NiFe2O4磁性纳米材料,其直径大小约为50 nm,比表面积38.15 m2·g-1,具有良好的结晶度、热稳定性、分散性以及较强的磁性.利用NiFe2O4磁性纳米材料NiFe2O4 MNPs表面的Ni2+能够与富组氨酸蛋白上的咪唑环通过配位键结合,以此来实现对富组氨酸蛋白的吸附.实验证明NiFe2O4磁性纳米材料能吸附天然富组氨酸蛋白质牛血红蛋白,吸附量可达到235 mg·g-1,高于文献报道的120.1 mg·g-1,还可以用于人血血红蛋白的吸附,具有实际应用价值.
Abstract
Introducing biocompatible and hydrophilic polymer material polyethylene glycol,NiFe2O4 magnetic nanomaterials were synthesized via a one-step hydrothermal method. The average diameter size of the nanomaterial was 50 nm and it had a specific surface area of 38.15 m2·g-1 with good crystallinity,thermal stability,dispersion and strong magnetic properties. Ni2+on the surface of NiFe2O4 MNPs could form coordination bonds with the imidazole ring of histidine-rich proteins,so as to bind with histidine-rich proteins. The results showed that NiFe2O4 magnetic nanomaterial could bind with natural histidine-rich protein bovine hemoglobin,the binding capacity was 235 mg·g-1,which was higher than 120.1 mg·g-1 reported in the literature. It could also be used for binding with hemoglobin in human blood,which has good practical application value.
关键词
蛋白质吸附/磁性纳米材料/富组氨酸蛋白质/双金属氧化物Key words
protein adsorption/magnetic nanoparticles/histidine-rich protein/bimetallic oxide引用本文复制引用
基金项目
吉林省科技发展计划(20230204090YY)
出版年
2024
NETL
NSTL
万方数据