Preparation of NiFe2O4 Magnetic Nanoparticles for Affinity Adsorption of Histidine-Rich Proteins
Introducing biocompatible and hydrophilic polymer material polyethylene glycol,NiFe2O4 magnetic nanomaterials were synthesized via a one-step hydrothermal method. The average diameter size of the nanomaterial was 50 nm and it had a specific surface area of 38.15 m2·g-1 with good crystallinity,thermal stability,dispersion and strong magnetic properties. Ni2+on the surface of NiFe2O4 MNPs could form coordination bonds with the imidazole ring of histidine-rich proteins,so as to bind with histidine-rich proteins. The results showed that NiFe2O4 magnetic nanomaterial could bind with natural histidine-rich protein bovine hemoglobin,the binding capacity was 235 mg·g-1,which was higher than 120.1 mg·g-1 reported in the literature. It could also be used for binding with hemoglobin in human blood,which has good practical application value.
protein adsorptionmagnetic nanoparticleshistidine-rich proteinbimetallic oxide
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