Design of Spectral Imaging System for Fluorescence Immune Analyzer
In order to meet the requirements of small volume,high resolution and multi-channel spectral imaging system for fluorescence immune analyzer,a spectral imaging system using reflective planar grating as the beam splitting element was proposed.According to the beam splitting principle of reflective grating,the combination of off-axis reflective structure and aspherical correction lens is used to realize the precise beam splitting of the light source to be examined.In this paper,the initial structure of the spectrometer was determined by the design requirements such as resolution and spectral range,and the structure was further simulated and optimized by using Zemax software.The simulation results show that the optical system can achieve the design goals of compact structure and high resolution in the multi-spectral range with a slit width of 25 μ m and a grating profile of 0.3.The wavelength resolution is better than 1.5nm,which provides an effective design scheme for the portability of fluorescence immune analyzer.
spectral imaging systemfluorescence immune analyzerCzerny-Turner structureoptical system design
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