To explore the influence on the removal of obstacle factors in continuous cropping soil treated by anaerobic soil disinfestation ( ASD) under different treating temperature,taking tomato continuous cropping soil as the research object,five soil treatments were set up as the following:control( CK)、treated by ASD at 10 ℃,20 ℃,30 ℃ and 40℃,respectively,which were all sealed and treated in bio-chemical incubator for 3 weeks. The results showed that soil pH values were significantly increased and EC values decreased by ASD treatment at different temperatures,and the contents of soil organic carbon ( TOC) and available K increased significantly. Effect on elim-ination NO-3 contents in soils treated by ASD were poor at low temperature (≤10 ℃) ,while the NH+4 contents in soils treated by ASD significantly increased at high temperature ( 40 ℃) . The number of bacteria in soils treated by ASD significantly increased at different temperatures.The number of fungi and Fusarium oxysporum in soils treated by ASD at high temperature(40 ℃) were significantly de-creased.In conclusion, the best effect occurred in soil treated by ASD at high temperature (40℃),and the contents of TOC and availa-ble K in soil treated by ASD were significantly increased at different temperatures.