The seeds of raphanus sativus were taken as test material,and hydroponic experiments were carried out in perti dishes.Three different light treatments were set up:full light(L1),semi-shade(L2),and darkness(L3).Five types of nitrogen fertilizers were set under each light treatment.The effects of concentrations(0,25,50,75,100 mg/L)on R.sativus seed germination and seedling growth were studied.The results showed that under dark conditions,applying nitrogen fertilizer at a suitable depth will increase the weight of seedlings,thus improving the quality of seedlings.It also proves the comprehensive and compensatory effects of ecological fac-tors in the environment.