HPLC-PDA Combined with Box-Behnken Response Surface Method to Optimize the Extraction Process of Propolis Tablets
This study establishes HPLC-PDA combined with Box-Behnken response surface method to optimize the extraction process of propolis tablets.Extraction time,solid-liquid ratio and methanol concentration were independent variables,and the total normalized val-ues of Chrysin and galangin contents were dependent variables.The chromatography was performed on Hypersil ODS2 C18 column(4.6 mm× 150 mm,5 μm),and the mobile phase consisted of methanol-0.1％phosphoric acid(58∶42)and the flow rate is 1.0 mL/min;The column temperature was 25 ℃ and the detection wavelength was 268 nm.The Box-Behnken response surface method was used to optimize the extraction process of propolis tablets and verify it.The optimal extraction process was 30 times 83％methanol extraction and the extraction time was 10 min.The optimal extraction process of Box-Behnken response surface method is stable and reliable,which can provide reference for extraction and quality control of propolis tablets.
万方数据
