In this study,various methods such as basic geophysical property test,particle size analysis test,XRD diffraction test,and SEM scanning test are used to analyze and study the subgrade loess at dif-ferent layers in different road sections.The results of the study show that due to the low content of clay minerals,fewer clay particles,poor cementation between soil particles,and less proportion of clay minerals involved in"aggregation"because of the serious subsidence of the subgrade,"aggregation"is weak and soil particles connot form stable structures.Therefore,under the condition of water seepage saturation and cyclic vibration load,the soil may have high compressibility,which harms the damage resistance of the subgrade soil.Thus,the uneven settlement of roadbeds is aggravated.This research provides an important basis for the disease mechanism of the railway line and the development of soil modification materials.