Quantitative Analysis of Paprika Oleoresin Carotenoid Metabolome Based on UPLC-MS/MS
To obtain a comprehensive understanding of the composition and content of carotenoids in pap-rika oleoresin,the researchers performed qualitative and quantitative analysis of them,before and after sa-ponification treatment by using ultra-high liquid chromatography coupled with tandem mass spectrometry(UPLC-MS/MS)based technique.The results showed that 41 carotenoids were detected in unsaponified paprika oleoresin,with 52.92％carotene content and 47.08％lutein content.In the saponified paprika o-leoresin,18 kinds of carotenoids were detected,with 43.14％carotene content and 56.86％lutein con-tent.The carotenoids with higher content in paprika oleoresin before and after saponification were all(E/Z)-phytoene,β-carotene,capsanthin,zeaxanthin and β-cryptoxanthin.These results indicated that paprika oleo-resin is rich in carotenoids and this research can provide more information on the composition and content of its carotenoids and some theoretical basis for the utilization and exploitation of paprika oleoresin.
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