基于UPLC-MS/MS对辣椒红类胡萝卜素代谢组的定量分析
Quantitative Analysis of Paprika Oleoresin Carotenoid Metabolome Based on UPLC-MS/MS
李远桥 1胡杰 2吴雯 3耿放 1王金秋 1吴笛4
作者信息
- 1. 成都大学食品与生物工程学院,四川 成都 610106
- 2. 成都大学机械工程学院,四川 成都 610106
- 3. 成都大学药学院,四川成都 610106
- 4. 成都大学食品与生物工程学院,四川 成都 610106;成都大学机械工程学院,四川 成都 610106;成都大学药学院,四川成都 610106
- 折叠
摘要
为了解辣椒红中类胡萝卜素的组成与含量,采用基于超高液相色谱与串联质谱联用技术(UPLC-MS/MS)对皂化处理前后辣椒红中的类胡萝卜素进行定性和定量分析.结果显示,未皂化的辣椒红检测出41种类胡萝卜素,其中,胡萝卜素含量占52.92％,叶黄素含量占47.08％;皂化后的辣椒红检测出18种类胡萝卜素,其中,胡萝卜素含量占43.14％,叶黄素含量占56.86％.皂化前后辣椒红中含量较高的类胡萝卜素均为八氢番茄红素、β-胡萝卜素、辣椒红素、玉米黄质和β-隐黄质等.结果表明,辣椒红具有丰富的类胡萝卜素,为了解辣椒红中类胡萝卜素的组成和含量提供了更多信息,对辣椒红的利用和开发提供了一定的理论依据.
Abstract
To obtain a comprehensive understanding of the composition and content of carotenoids in pap-rika oleoresin,the researchers performed qualitative and quantitative analysis of them,before and after sa-ponification treatment by using ultra-high liquid chromatography coupled with tandem mass spectrometry(UPLC-MS/MS)based technique.The results showed that 41 carotenoids were detected in unsaponified paprika oleoresin,with 52.92％carotene content and 47.08％lutein content.In the saponified paprika o-leoresin,18 kinds of carotenoids were detected,with 43.14％carotene content and 56.86％lutein con-tent.The carotenoids with higher content in paprika oleoresin before and after saponification were all(E/Z)-phytoene,β-carotene,capsanthin,zeaxanthin and β-cryptoxanthin.These results indicated that paprika oleo-resin is rich in carotenoids and this research can provide more information on the composition and content of its carotenoids and some theoretical basis for the utilization and exploitation of paprika oleoresin.
关键词
辣椒红/类胡萝卜素/UPLC-MS/MS/皂化Key words
paprika oleoresin/carotenoid/UPLC-MS/MS/saponification引用本文复制引用
基金项目
四川省自然科学基金(2023NSFC0183)
出版年
2024
国家科技期刊平台
NSTL
万方数据