Study on Extraction,Purification,Stability and Anti-Oxidant Abilities of Quinoa Betalains
By using the above-ground part of quinoa material ZL-7 at its maturity stage as the experimental material,this study explored various extraction conditions for quinoa betalains,including extraction sol-vent,solid-liquid ratio,temperature,time and rotation speed.The optimal extraction process of betalains was obtained by response surface optimization.Betalains were purified by ethanol dehydration and macro-porous resin adsorption.The effects of light,pH and temperature on betalains'stability and antioxidant ca-pacity were investigated.The results showed that the optimized extraction solvent was deionized water,the solid-liquid ratio was 1:20(g/mL),the temperature was 27 ℃,the extraction time was 35 min,and the shaking speed was 150 r/min.Finally,the actual yield of betalains was 9.55％±0.12％,and the purity was 87.57％±1.60％.The adsorption effect of macroporous resin on quinoa betalains was XDA-7＞HP20＞D101＞S-8＞NKA＞AB-8,the analytical effect was D101＞HP20＞AB-8＞XDA-7＞NKA-9＞S-8.The results showed that D101 was the best resin for the purification of quinoa betalains,and the plain color value of quinoa betalains could be increased by 5.72％±1.13％byD101 adsorption.Stability ex-periments showed that betalains were the most stable under the conditions of light avoidance,temperature below 30 ℃,and pH 3.0-11.0.The antioxidant experiment showed:the quinoa betalains ABTS clearance rate＞DPPH clearance rate＞OH clearance rate of betalains.
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