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激素对银杏叶愈伤组织状态的影响及悬浮培养体系的建立

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以银杏叶片为材料,研究生长素萘乙酸(NAA)与2,4-二氯苯氧乙酸(2,4-D),以及细胞分裂素6-苄氨基腺嘌呤(6-BA)和激动素(KT)的不同组合、不同配比,以及培养基类型对愈伤组织状态的影响,以此筛选出适合银杏叶愈伤组织诱导的最佳激素组合和培养基,优良的愈伤组织经继代培养后,能够建立银杏悬浮细胞培养体系.结果表明,NAA和6-BA组合、NAA和KT组合的诱导率均高于2,4-D和6-BA组合、2,4-D和KT组合,且诱导出的愈伤组织状态较优良;不同激素组合诱导的愈伤组织褐变程度不同,NAA和6-BA组合、NAA和KT组合诱导的愈伤组织不发生褐变,2,4-D和6-BA组合、2,4-D和KT组合诱导的愈伤组织褐变程度较严重;NAA和KT组合时,NAA浓度为1.5 mg/L,KT浓度为1.0~1.5 mg/L时,诱导率高,愈伤组织的状态优良;NAA和6-BA组合时,NAA浓度为1.5 mg/L,6-BA浓度为0.5~1.0 mg/L时,愈伤组织的诱导率高;诱导银杏叶愈伤组织适合的培养基是N6和MS.结果显示,NAA与KT、NAA与6-BA这2种激素组合在N6或MS培养基上均能诱导出优良的愈伤组织(疏松、颗粒状、黄绿色、无褐变与无水渍状),经继代培养后,可建立起银杏悬浮细胞培养体系.
Effect of Hormones on Callus Status of Ginkgo Biloba Leaves and Establishment of Suspension Culture System
By using Ginkgo Biloba leaves as materials,the effects of different combinations and proportions of auxin(NAA,2,4-D),cytokinin(6-Ba,KT)and different culture media on callus state were studied so as to screen out the best combination of hormones and culture media suitable for callus induction of Gink-go Biloba leaves.Excellent suspension cell culture system of Ginkgo Biloba was established after the sub-culture of fine callus.The results showed that the callus induction rate of NAA and 6-BA combination,NAA and KT combination was higher than that of 2,4-D and 6-BA combination,2,4-D and KT combina-tion.And the induced callus state was better.The browning degree of callus induced by different hormone combinations was different.The callus induced by NAA and 6-BA combination,NAA and KT combination had no browning,and the browning degree of 2,4-D and 6-BAcombination,2,4-D and KT combination was more serious.When NAA and KT was combined,NAA concentration was 1.5 mg/L and KT concen-tration was 1.0~1.5 mg/L,the induction rate of callus was high and the callus state was excellent.When NAA and 6-BA was combined,and NAA concentration was 1.5 mg/L and 6-BA concentration was 0.5~1.0 mg/L,the callus induction rate was high.The suitable culture medium for inducing Ginkgo Biloba leaves callus were N6 and MS.In conclusion,the suitable combinations of hormones for inducing Ginkgo Biloba callus are NAA and KT,NAA and 6-BA.Excellent callus(loose,granular,yellow-green,no brown-ing,no water stain)can be induced by the combinations on N6 or MS culture medium.After subculture,the suspension cell culture system of Ginkgo Biloba can be established.

Ginkgo Bilobacallusstatussuspension culturebrowning

李玲玲

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重庆工贸职业技术学院健康学院,重庆 408000

银杏叶 愈伤组织 状态 悬浮培养 褐变

2024

成都大学学报(自然科学版)
成都大学

成都大学学报(自然科学版)

影响因子:0.357
ISSN:1004-5422
年,卷(期):2024.43(4)