Effects of high pH stress on acute toxicity and SOD activity in Procambarus Clarkii
pH is an important index to measure water quality, Too high or too low pH of water will be harmful to aquatic organisms. In order to understand the influence of water pH on Procambarus clarkii, and provide a basis for Procambarus clarkii breeding and production, in the present study, five gradients were set from pH 8-12 with the range of 1.0, and recorded the mortality of Procambarus clarkii at 24, 48, 72 and 96 h respectively. On the basis of 96 h LC50 (9.71), the control group (pH 7.6) and the experimental group (pH 9.7) were set up for 96 h high pH stress, and SOD activity of blood was measured at 0, 2, 8, 24 and 96 h after stress. The results showed that the death of Procambarus clarkii was different at each time point in the 5 gradients of pH 8-12. the mortality of Procambarus clarkii was 0 at each time point at pH 8, and no Procambarus clarkii died in 24 h pH 9. While, the mortality rate increased with time, the death rate of Procambarus clarkii was 16.50％at 24 h and increased to 53.00％at 96 h at pH 10. The death rate of Procambarus clarkii reached 64.00％ at 24 h and 100％ at 48 h at pH 11. The mortality of Procambarus clarkii was 100％ at 24 h at pH 12. The SOD activity of Procambarus clarkii blood have no significantly changes at different time point in the control group, but decreased with time in all experimental groups. There was no significant difference between the experimental group and the control group at 0-8 h. While, there was significant difference at 24 h and 96 h (P<0.05).
万方数据
维普
