To create elite germplasms and accelerate the process of breeding new cultivars in Sorghum bicolor X Sorghum sudanense, the 'pacesetter' cultivar induced by space flight was evaluated based on morphological characteristics and SSR markers. Most agronomic traits showed a growth tendency from SP1to SP4 generation then were able to keep stable at SP4 generation based on four years observation in field. Finally, nine elite lines were selected for further study. Meanwhile, ten pairs of SSR primers from Sorghum were selected for SSR analysis of different variations selected from SP1 ~SP4 generation of Sorghum bicolor X Sorghum sudanense. A total of 203 bands were detected, of which 185 bands were polymorphic with a polymorphic rate of 91. 1%. A rich genetic diversity had been revealed in the induced materials, and a good transferability of SSR markers form Sorghum had also been revealed in Sorghum bicolor X Sorghum sudanense. Cluster analysis showed that the same kinds of variation in the same generation could be grouped together. But there was incomplete correlation between SSR markers and morphological characteristics. It might be caused by the complex genetic control of characteristics or material selected from the same or similar lines. These results will provide useful information for creating new elite germplasms and breeding in Sorghum bicolor X Sorghum sudanense.