Diagnostic Value and Molecular Mechanism of LASP-1 in the Process of Advanced Adenoma and Carcinogenesis
Objective To detect the expression level of LASP-1 in advanced adenoma and adenocarcinoma and its diagnostic value,and to explore the molecular mechanism of malignant transformation of advanced adenoma.Methods Plasma samples were collected from 48 patients with colorectal adenocarcinoma(CRC group),13 patients with adenoma(CRA group)and 22 healthy volunteers(Normal group).The expression level of LASP-1 in plasma samples was detected by enzyme-linked immunosorbent assay(ELISA).The expression of CEA in plasma was detected by automatic chemiluminescence immunoassay.The diagnostic efficacy of plasma LASP-1,CEA and their combination in CRC and CRA was evaluated by the area under the ROC curve.Spearman correlation analysis was used to analyze the relationship between plasma LASP-1 and clinicopathological features.The expression of LASP-1 in CRC stage Ⅰ-Ⅳ patients and Normal group was detected in TCGA database.Next,the differential expression of LASP-1 in CRC and CRA groups was detected in GEO database.Immunohistochemical method and human protein immunohistochemistry database were used to detect the expression of P53 and LASP-1 in CRC tissues.Results ELISA results showed that the plasma concentrations of LASP-1 in CRC and CRA groups were significantly higher than those in Normal group(P<0.01),while there was no significant difference in CEA expression levels among CRC,CRA and Normal groups.ROC curve analysis showed that the sensitivity and specificity of plasma LASP-1 in the diagnosis of CRC and CRA were higher than those of CEA.When the two were combined,the area under the curve(AUC)was 0.982 and 0.99,respectively.TCGA database results showed that the expression level of LASP-1 in the tissues of CRC patients in each stage was significantly higher than that in the Normal group(P<0.05).GEO database results showed that the expression level of LASP-1 in the CRC group was significantly higher than that in the CRA group(P<0.05).Immunohistochemical results showed that mutant P53 accounted for 64.5％of the CRC group.Human protein immunohistochemistry database showed that LASP-1 was highly expressed in CRC tissues compared with normal intestinal epithelial tissues.Conclusion LASP-1 combined with CEA is a potential diagnostic indicator for early diagnosis of CRC and CRA patients;LASP-1 may be involved in the molecular mechanism of adenomasis cancer,making it possible for CRC to be detected and intervened in the adenoma stage.
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维普
