Protective Effect of Vestitol on Human Umbilical Vein Endothelial Cells Injury Induced by Hydrogen Peroxide
Objective To investigate the protective effect of vestitol on human umbilical vein endothelial cells(HUVEC) injury induced by hydrogen peroxide (H2O2).Methods HUVEC were cultured in vitro,and a series of concentrations of H2O2 at 700～900μmol/L were set to screen the damaged concentration of H2O2 to HUVEC.A series of concentrations of vestitol at 1.25～20mg/L were set up to screen the cytotoxic effect of vestitol on HUVEC.HUVEC were exposed to H2O2 to evaluate the protective effect of vestitol on HUVEC injury induced by H2O2.Ginkgo biloba extract (GBE) was used as the positive control drug.The cell morphology was observed by the inverted microscope,the cell viability was detected by CCK-8 method and the lactate dehydrogenase (LDH) release was detected by the pyruvate reduction method.Results Along with the increase of H2O2 concentration,the damage degree of HUVEC gradually increased,the cell morphology was injured,the cell viability gradually decreased,and the LDH release gradually increased.The damaged concentration of H2O2 to HUVEC was determined to be 750 μmol/L.1.25～10mg/L vestitol had no cytotoxicity to HUVEC,20mg/L vestitol began to produce toxic effects on HUVEC,and the highest non-toxic concentration was 10mg/L.Vestitol can improve the abnormal morphological changes of HUVEC caused by H2O2,increase the cell viability of HUVEC,and reduce the release of LDH.Conclusion Vestitol can effectively reduce HUVEC damage caused by H2O2.
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