Sericin Targeting Akt1 Regulates PI3K/Akt Signaling Pathway to Promote Proliferation of INS-1 Cells Damaged by STZ
Objective To investigate whether sericin promotes proliferation of streptozotocin (STZ) damaged insulinoma cells (INS-1 cells) through targeting Akt1 regulates PI3K/Akt signaling pathway.Methods INS-1 cells were randomly divided into four groups.In control group,INS-1 cells were cultured under conventional conditions without other treatments.In model group,INS-1 cells were cultured with 10mmol/L STZ.In sericin group,INS-1 cells were cultured with 10mmol/L STZ and 600μg/mL sericin.In inhibitor group,INS-1 cells were cultured with 10mmol/L STZ,600μg/mL sericin and 0.3mmol/L Akt1 inhibitor A-674563.The cells in four groups were cultured with corresponding drugs respectively for 24h.The survival rate of INS-1 cells in each group was detected by CCK-8 method.Western blot was used to detect the expression of PI3K/Akt signaling pathway related proteins PI3K and p-Akt,and proliferation related proteins PCNA and Ki67.Results The survival rate,and the protein expressions of PI3K,p-Akt1,PCNA,and Ki67 of INS-1 cells in model group significantly decreased compared with control group (P<0.05).The survival rate,and the protein expressions of PI3K,p-Akt1,PCNA,and Ki67 of INS-1 cells in sericin group significantly increased compared with model group (P<0.05).The survival rate,and the protein expressions of PI3K,p-Akt1,PCNA,and Ki67 of INS-1 cells in inhibitor group significantly decreased compared with sericin group (P<0.05).Conclusion Sericin can protect proliferation of INS-1 cells damaged by STZ,and the protective mechanisms may related to target Akt1 regulates PI3K/Akt signaling pathway.