Regulatory Effects of Sericin on Glycolysis of Damaged INS-1 Cells through PI3K/Akt Signal Pathway
Objective To observe the regulatory effects of sericin on PI3K/Akt signal pathway and glycolysis of INS-1 cells injured by streptozotocin(STZ).Methods INS-1 cells cultured in vitro were randomly divided into five groups.Normal control group,model group,sericin group,Akt1 inhibitor group and Akt1 agonist group.Western blotting and real-time fluorescence quantitative PCR were used to detect the expression of phosphatidylinositol-3-kinase(PI3K),protein kinase B(Akt),phosphofiuctosekinase-1(PFK1),6-phosphofructose-2,6-diphosphatase(PFKFB2)protein and mRNA.Results Compared with the normal control group,the protein expression of PI3K,p-Akt,PFK1,PFKFB2 of INS-1 cells in the model group decreased significantly(P＜0.05).The protein expression of PI3K,p-Akt,PFK1,PFKFB2 of INS-1 cells in the sericin group were significantly higher than that in the model group(P＜0.05).The protein expression of p-Akt,PFK1,PFKFB2 of INS-1 cells in the Akt1 inhibitor group were significantly lower than that in the sericin group(P＜0.05).Compared with sencin group,the protein expression of p-Akt,PFK1 and PFKFB2 showed an upward trend in the Akt1 agonist group.The change trend of PI3K,Akt,PFK1,PFKFB2 mRNA expression in INS-1 cells of each group were consistent with that of protein.Conclusion The protective mechanism of sericin on STZ-induced injury of INS-1 cells may be that targeted Akt1 affects PI3K/Akt signal pathway and enhances glycolysis.
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维普
