氯霉素和土霉素可视化适配体检测试纸研究与制备
Research and Preparation of Visual-Paper Aptasensor for Oxytetracycline and Chloramphenicol
王红旗 1郑嘉 1刘继红 1王俊艳 1曹成 1尹海燕 1刘雪平 2王冕3
作者信息
- 1. 河南省农业科学院农业质量标准与检测技术研究所,河南 郑州 450002
- 2. 河南城建学院市政与环境工程学院,河南 平顶山 467000
- 3. 唐河县农业农村局,河南 南阳 473400
- 折叠
摘要
为实现多抗生素的便捷、可视化检测,本文以核酸适配体作为分子识别元件,采用ISM技术进行定向进化,利用碳点纳米材料作换能器,采用链置换信号传导技术和表面临近杂交信号的输出策略,研制出了氯霉素和土霉素可视化检测试纸.结果显示,氯霉素和土霉素可视化适配体检测试纸是一种signal-on比色型传感器,检测带的亮度与靶标的浓度正相关.经液质联用法确证,该检测试纸的灵敏度为:土霉素≥24.6 μg/L,氯霉素>10.26 μg/L.实验结果证明该检测试纸具有经济、快捷、高特异性的优点,有望为政府部门的抗生素风险评估、滥用监管和基层用户的质量控制提供技术支撑.
Abstract
To realize convenient and visual detection of multiple antibiotics,visual test strip for detection of chloramphenicol and oxytet-racycline is developed using aptamer as molecular recognition element,ISM technology for directed evolution,carbon point nanomaterials as transducers and then integrating signal transmission technology of chain displacement and signal output strategy of surface near the hybridization.Experimental results show that the aptasensor proposed is a signal-on type test paper utilizing colorimetric principle,and the brightness of the test line shows positive correlation with the concentration of the targets.Confirmed by LC-MS/MS,the sensitivity of oxytetracycline is≥24.6 μg/L,and that of chloramphenicol is>10.26 μg/L.In conclusion,this aptasensor has the advantages of econ-omy,efficiency and high specificity,and is expected to provide technical support for the quality control of primary users and the risk as-sessment and abuse supervision of antibiotics by government.
关键词
适配体传感器/可视化检测/检测试纸/抗生素/土霉素/氯霉素Key words
aptasensor/visual detection/test strip/antibiotics/oxytetracycline/chloramphenicol引用本文复制引用
基金项目
河南省农业科学院自主创新专项项目(2020ZC47)
出版年
2024
国家科技期刊平台
NSTL
万方数据