Research and Preparation of Visual-Paper Aptasensor for Oxytetracycline and Chloramphenicol
To realize convenient and visual detection of multiple antibiotics,visual test strip for detection of chloramphenicol and oxytet-racycline is developed using aptamer as molecular recognition element,ISM technology for directed evolution,carbon point nanomaterials as transducers and then integrating signal transmission technology of chain displacement and signal output strategy of surface near the hybridization.Experimental results show that the aptasensor proposed is a signal-on type test paper utilizing colorimetric principle,and the brightness of the test line shows positive correlation with the concentration of the targets.Confirmed by LC-MS/MS,the sensitivity of oxytetracycline is≥24.6 μg/L,and that of chloramphenicol is>10.26 μg/L.In conclusion,this aptasensor has the advantages of econ-omy,efficiency and high specificity,and is expected to provide technical support for the quality control of primary users and the risk as-sessment and abuse supervision of antibiotics by government.
国家科技期刊平台
NSTL
万方数据
