Design of microfluidic PCR detection system based on embedded ARM
In order to realize the integration of microfluidic equipment functions and improve the operability of interactive system,a design scheme of microfluidic polymerase chain reaction(PCR)control system and host computer software based on embedded ARM is proposed.The system hardware adopts multiple embedded ARM controllers to coordinate with the functional modules,formulate scalable communication mechanism and protocol,and realize the integrated control of flow path motion,temperature control,fluorescence signal acquisition and other functions in the process of microfluidic PCR detection.Using Qt and SQLite database,the host computer software is designed.Using multithreading and node mapping,the coordinated control of hardware modules and the storage of detection information are realized.Experiments show that the system runs stably,has good human-computer interaction effect and high operability,meets the integrated functional requirements of microfluidic PCR.