Design and optimization of sperm optimization chip based on silicon-based filter membrane
The sperm optimized microfluidic chip based on silicon-based filter membrane is constructed by using semiconductor technology.Sperm with high vitality will overcome gravity and move downstream from the micropore to the sperm collection pool.Sperm samples in the collection pool are collected at 7,15,30 and 60 minutes,respectively.Routine semen detection,sperm morphology and DNA fragment analysis are performed using CASA system.The pore spacing and screening time of silicon-based filter membrane are optimized.Results show that 6 μm pore spacing and 15minutes screening time are the best sperm optimization scheme.The constructed sperm optimization chip based on silicon-based filter membrane can effectively select sperm with high vitality,DNA integrity and high normal morphology.Semen throughput of chip is high,mobile sperm recovery rate is high,it has certain clinical value.At the same time,a simple and easy to operate standardized sperm optimization process is provided.
万方数据
维普
