Precision micromanipulation has important application value in advanced life science such as single cell analysis.Capillary tip is a widely-used end effector of micromanipulator.Whether it can accurately measure and control the caliber of the capillary tip directly affects the precision and reliability of the end application.A capillary tip caliber recognition algorithm based on computer vision is proposed.This algorithm can adaptively determine the region of interest(ROI)to accurately obtain the position of the capillary tip.On this basis,a bidirectional synchronous point scanning strategy is adopted to locate the point at the end of the capillary tip and the caliber of capillary tip can be calculated.The experimental results show that when the proposed method is applied to capillary caliber detection on a micro manipulation platform,the caliber identification error is less than 1 μm,meeting the precision requirements for single cell analysis scene.This method overcomes the problems of traditional algorithms which relies on templates or backgrounds,and low accuracy of caliber detection,laying a good algorithm foundation for precision micromanipulation technology.
关键词
精密显微操控/毛细针/口径识别/感兴趣区域/单细胞分析
Key words
precision microscopic control/capillary needle/caliber identification/region of interest(ROI)/single cell analysis
维普
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