Design of intrinsic fluorescence counting instrument for aerosol microbial particles
Aiming at the demand for real-time monitoring of aerosol microbial particles,the detection methods and devices based on microbial particles intrinsic fluorescence and light scattering are studied.The basic principle of particle light scattering detection is analyzed.Then,the fluorescence spectra of microbial particles flourescent active molecules and Staphylococcus aureus and other six microorganisms are tested and analyzed.The overall design schemes of 405 nm excitation light and two-way scattered light are determined.Through the simulation of the important parameters through the gas path and the detection cavity structure,the optimal design is realized.Finally,the construction of the detection device is completed.The preliminary test results show that the device realizes the synchronous detection of particle scattered light and fluorescence,and can distinguish and count ordinary dust particles and microbial particles,which lays a foundation for further research and development of real-time monitoring instrument for aerosol microbial particle concentration.
万方数据
维普
