Research on electrochemical sensor for in vivo detection of indole-3-acetic acid
As an important plant hormone,indole-3-acetic acid(IAA)plays a crucial role in the whole process of plant growth and development.According to the actual needs of in vivo detection of plants,a four-array electrode suitable for plant stem and a six-array electrode for plant fruit are developed.Platinum nanoparticles(PtNPs)and reduced graphene oxide(rGO)nanocomposite film are deposited on the platinum wire working electrode by one-step method,thereby enhancing the catalytic effect and improving the sensitivity of the biosensor.The experimental results show that the developed sensor has a good linear relationship between the IAA concentration range of 0.1～200μmol·L-1,correlation coefficient R2 =0.986,and limitation of detection is 0.01μmol·L-1(S/N =3).The prepared sensor is further used to detect the IAA content in cucumber stem and cucumber fruit in vivo,indicating that the sensor has important application prospects in the in vivo detection of plant physiological states.
in vivoindole-3-acetic acid(IAA)electrochemicalsensor
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