Single cell analysis is of great significance in biomedical research and clinical medicine applications. Microfluidic chip is an effective tool for single cell analysis. In order to achieve efficient capture and real-time observation of single cell,a single-row ring microfluidic chip is designed based on fluid mechanics. Four different sizes(15,20,25,30μm)of back-end channels are designed for each capture chamber. Velocity flow field analysis of single-row ring microfluidic chip is carried out by fluent software. Simulation results show that the microfluidic chip with the back-end channel size of 15μm and 20μm can achieve the best capture effect. A microfluidic chip with a back-end channel of 20μm is used to capture single polystyrene particles. Experimental results show that the capture efficiency of single-row ring microfluidic chip can reach up to 91. 67％,which provides a research basis for subsequent cell capture and cell culture.
关键词
单细胞分析/粒子捕获/细胞捕获/流场分析/细胞培养
Key words
single cell analysis/particle capture/cell capture/flow field analysis/cell culture
维普
万方数据