Research and fabrication of point-of-care testing microfluidic system for pathogen of urinary tract infection
In order to achieve rapid diagnosis on site,a system which can achieve point-of-care test(POCT)of urinary tract infection pathogen is researched and fabricated based on the centrifugal microfluidic technology.The system is composed of centrifugal detection chip,selector valve injection chip and POCT device.Take advantage of the theory of colorimetric reaction,the result can be observed by naked eyes within 60 minutes.The quantitative results of experiment suggest that the device can reach a recovery rate of E.coli to 99.8％ in a certain range,and the limitation of detection is 105 CFU/mL.At last,four-groups of simulattion samples with concentration gradients of 105～108 CFU/mL E.coli is detected and the testing results corresponding to the four groups concentrations are recorded.These results are translated to a standard colorimetric card for further analysis of detection result.This system has the advantages of low cost,easy to operate and fast detection,has a certain application value.
urinary tract infectionpathogen enrichmentcentrifugal microfluidicpoint-of-care test
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