Trypsin sensor in human urine based on enzyme-functionalized quantum dots
Glucose oxidase (GOD )-functionalized Mn-doped ZnS GOD-Mn-ZnS quantum dots (QDs ),are prepared using GOD as the template. The synthesis conditions of QDs are optimized by orthogonal experiments.Then a phosphorescence sensing system for detecting trypsin is constructed,the quantitative detection of trypsin in human urine is realized. The phosphorescent quenching degree of QDs show a good linear relationship with the concentration of trypsin. The linear range is 5～100 mg/L,R2=0.998,and the detection limit is 2.21 mg/L. The urine of healthy volunteers is detected by standard addition method,and the recovery rate is 97.45％～103.00％. The method can be used for rapid detection of trypsin in urine of patients with acute pancreatitis and has good biomedical application prospects.
万方数据
维普
