目的 利用磁共振功能成像非对称回波最小二乘估算法迭代水脂分离(iteraterative decomposition of water and fat with echo asymmetry and least-squares estimation quantitation,IDEAL-IQ)技术无创定量评估 2 型糖尿病(type 2 diabetes mellitus,T2DM)大鼠肝脏、肾脏及胰腺的脂肪沉积与铁沉积,并研究T2DM大鼠空腹血糖(fasting blood glucose,FBG)、体质量与脂肪沉积、铁沉积之间的关系,同时比较实验组和对照组大鼠肝肾功、血脂以及病理学改变的差异.材料与方法 10只无特定病原体(specific pathogen free,SPF)健康雄性SD大鼠随机进行分组为实验组(n=7)和对照组(n=3).实验组进行T2DM的模型建立.待实验组成模后,将两组大鼠进行IDEAL-IQ扫描,通过测定两组大鼠的肝脏、胰腺及肾脏的质子密度脂肪分数(proton density fat fraction,PDFF)和横向弛豫率(transverse relaxation rate,R2*)来评估实验组以及对照组大鼠肝脏、胰腺及肾脏的脂肪沉积及铁过载,扫描结束后心脏取血评估肝功、肾功及血脂改变.处死大鼠后取肝脏、肾脏及胰腺做常规HE染色观察细胞的变化、油红O染色观察脂肪沉积、普鲁士蓝铁染色观察铁沉积.实验数据运用SPSS 27.0软件进行统计学分析,通过Pearson相关系数分析大鼠FBG、体质量与各器官PDFF、R2*值之间的相关性.结果 T2DM组SD大鼠的FBG、体质量、PDFF胰腺、PDFF肝脏、PDFF右肾、PDFF左肾、R2*胰腺、R2*肝脏、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)均高于对照组,差异有统计学意义(P<0.05),但两组大鼠在T1 SI胰腺、T1 SI肝脏、T1 SI右肾、T1 SI左肾、T2 SI胰腺、T2 SI肝脏、T2 SI右肾、T2 SI左肾、R2*右肾、R2*左肾、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、尿素氮(BUN)、肌酐(Cr)等方面比较差异没有统计学意义(P>0.05).Pearson相关性分析,FBG与PDFF肝脏(r=0.773)、PDFF胰腺(r=0.837)、PDFF右肾(r=0.895)、PDFF左肾(r=0.784)、R2*肝脏(r=0.876)、体质量(r=0.980)均呈正相关(P<0.05).体质量与PDFF胰腺(r=0.840)、PDFF右肾(r=0.854)、PDFF左肾(r=0.796)、R2*肝脏(r=0.834)、R2*胰腺(r=0.778)均呈正相关(P<0.05).结论 本实验通过利用MRI IDEAL-IQ技术无创定量评估T2DM大鼠肝脏及胰腺脂肪沉积与铁沉积的含量,并且同时评估了两组大鼠双肾的脂肪含量差异.这项技术有望动态随访新诊断的糖尿病患者,早期评估其肝脏、肾脏以及胰腺脂肪含量和铁含量改变,为指导临床诊断及治疗提供新的方向.
Application of IDEAL-IQ to quantitatively evaluate fat deposition and iron overload in abdominal parenchymal organs in rats with type 2 diabetes mellitus
Objectives:The MRI iteraterative decomposition of water and fat with echo asymmetry and least-squares estimation quantitation(IDEAL-IQ)technique was utilized to non-invasively and quantitatively assess fat deposition and iron deposition in the liver,kidney and pancreas of rats with type 2 diabetes mellitus(T2DM),as well as to study the relationship between fasting blood glucose(FBG),body weight,and fat deposition and iron deposition in T2DM rats,and to observe the laboratory and pathological alterations between groups.Materials and Methods:Ten specific pathogen free(SPF)healthy male SD rats were randomly grouped into subgroups,experimental group(n=7)and control group(n=3).The experimental group was subjected to the establishment of a model of T2DM,after the experimental group was modeled,the two groups of rats were scanned with MRI IDEAL-IQ.The proton density fat fraction(PDFF)and transverse relaxation rate(R2*)of the liver,pancreas and kidney of the two groups of rats were measured to evaluate the fat deposition and iron overload in the liver,pancreas and kidney of the experimental group and the control group,and to assess the changes in liver function,renal function,and lipids by blood sampling from the heart at the end of the scanning process.The liver,kidney,and pancreas were taken at execution for routine HE staining to observe cellular changes,oil red O staining to observe fat deposition,and Prussian blue iron staining to observe iron deposition.The experimental data were statistically analyzed using SPSS 27.0 software,and the Pearson correlation coefficient was used to analyze the correlation between FBG,body weight and PDFF and R2* values of various organs in rats.Results:The FBG,body weight,triglycerides(TG),and low-density lipoprotein cholesterol(LDL-C)of SD rats in the T2DM group were higher than those of the control group,and the PDFF of the pancreas,liver,right kidney,and left kidney as well as the R2* of the pancreas and liver were higher than those of the control group,and the differences were statistically significant(P<0.05).However,the differences in T1 signal intensity and T2 signal intensity of the pancreas,liver,and both kidneys were not statistically significant between the two groups of rats,and the differences in R2*,total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C),aspartate transaminase(AST),alanine aminotransferase(ALT),blood urea nitrogen(BUN),and creatinine(Cr)of both kidneys were not statistically significant when compared with those of the control group(P>0.05).Pearson's correlation analysis showed that the differences between FBG and PDFF in the liver(r=0.773),PDFF of the pancreas(r=0.837),PDFF of the right kidney(r=0.895),PDFF of the left kidney(r=0.784),R2* of the liver(r=0.876),and body weight(r=0.980)were positively correlated(P<0.05).Body weight was positively correlated with PDFF of the pancreas(r=0.840),PDFF of the right kidney(r=0.854),PDFF of the left kidney(r=0.796),PDFF of the liver(r=0.834),and PDFF of the pancreas(r=0.778)(P<0.05).Conclusions:In this experiment,MRI IDEAL-IQ technology was used to non-invasively and quantitatively evaluate the content of fat deposition and iron deposition in the liver and pancreas of T2DM rats,and the difference in fat content in both kidneys of the two groups of rats was also evaluated.This technique is expected to provide a new direction for clinical diagnosis and treatment by dynamically following newly diagnosed diabetes mellitus patients,and assessing changes in liver,kidney,and pancreatic fat and iron content at an early stage.
magnetic resonance imagingIDEAL-IQratstype 2 diabetes mellitusfat depositioniron deposition
万方数据
维普
