Effect of ectopic expression of SMAF1 on proliferation and apoptosis of preadipocyte 3T3-L1
Objective To evaluate the effect of a novel adipocyte-specific expressed gene small adipocyte factor 1 (SMAF1) on proliferation and apoptosis of preadipcyte 3T3-L1. Methods A permanent SMAF1 expression construct was established for stable transfection of 3T3-L1 and formation of SMAF1 3T3-L1 cell line. After invitro cul-turing of cells, both MTT method and flow cytometry were used to detect and analyze the proliferation level and apoptosis progress respectively. Results Compared with control groups, SMAF1 3T3-L1 cell line showed remarkable reduction in proliferation with statistical significance (P<0. 05), while no notable difference in apoptosis between two cell lines. Conclusion The ectopic expression of SMAF1 in 3T3-L1 preadipocyte might suppress proliferation without interfering apoptosis process.
small adipocyte factor 1preadipocytesectopic expressionproliferationapoptosis
NETL
NSTL
万方数据
维普
