Effect of SF3B1/FOXM1/JUNB Axis Regulating SOX21 Expression on Biological Behavior of Cervical Cancer Cells
Objective To analyze the effect of splicing factor 3B subunit 1/forkhead box M1/transcription factor activated protein kinase B(SF3B1/FOXM1/JUNB)axis regulated expression of transcription factor 21 antibody(SOX21)on the biological behavior of cervical cancer cells.Methods The para-cancerous tissues and cancerous tissues of 50 cervical cancer patients admitted to the Affiliated Cancer Hospital of Xinjiang Medical University from March 2022 to December 2023 were selected as the study objects.The expression levels of SF3B1,FOXM1,JUNB and SOX21 were detected by real-time fluorescence quantitative PCR.The biological behaviors of cervical cancer cells(proliferation,migration and invasion)were detected by Transwell and cell counting kit-8(CCK8).The protein expressions of SF3B1,FOXM1,JUNB and SOX21 were determined by Western blot.Results Compared with the adjacent tissues,the cervical cancer tissues showed a lower expression level of JUNB and higher expression levels of FOXM1,SOX21,and SF3B1,and the differences were statistically significant(P＜0.05).Compared with the si-NC group,the si-SF3B1/FOXM1/JUNB group exhibited a higher optical density(OD)450 value at 0 h,but lower invasive cell count,migratory cell count,(24,48 h)OD450 values,SF3B1,FOXM1,and JUNB,and the differences were statistically significant(P＜0.05).Compared with the OE-NC group,the OE-SOX21 group showed higher migratory cell count,(0,24,48 h)OD450 values,SOX21,and invasive cell count,and the differences were statistically significant(P＜0.05).Compared with the si-SF3B1/FOXM1/JUNB+OE-NC group,the si-SF3B1/FOXM1/JUNB+OE-SOX21 group exhibited higher levels of SOX21,SF3B1,FOXM1,JUNB,(0,24,48 h)OD450 values,invasive cell count,and migratory cell count,and the differences were statistically significant(P＜0.05).Conclusion SF3B1/FOXM1/JUNB axis can promote the invasion,proliferation and migration of cervical cancer cells by activating SOX21 expression.
Splicing factor 3B subunit 1/forkhead box M1/transcription factor activated protein kinase BAntibody to transcription factor 21Cervical cancer cellMigrationInvasionProliferation
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