首页|Reconstruction of tyrosol synthetic pathways in Escherichia coli

Reconstruction of tyrosol synthetic pathways in Escherichia coli

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Tyrosol is a pharmacologically active phenolic compound widely used in the medicine and chemical industries.Traditional methods of plant extraction are complicated and chemical synthesis of tyrosol is not commercially viable. In this study, a recombinant Escherichia coli strain was constructed by overexpressing the phenylpyruvate decarboxylase ARO10 from Saccharomyces cerevisiae, which could produce tyrosol from glucose. Furthermore, genes encoding key enzymes from the competing phenylalanine and tyrosine synthesis pathways and the repression protein TyrR were eliminated, and the resulting engineered strain generated 3.57 mmol·L-1 tyrosol from glucose. More significantly, codon optimization of ARO10 increased expression and tyrosol titer. Using the novel engineered strain expressing codon-optimized AR10 in shake-flask culture, 8.72 mmol·L-1 tyrosol was obtained after 48 h. Optimization of the induction conditions improved tyrosol production to 9.53 mmol·L-1(1316.3 mg·L-1). A higher titer of tyrosol was achieved by reconstruction of tyrosol synthetic pathway in E. coli.

TyrosolEscherichia coliPhenylpyruvate decarboxylaseGene knockoutCodon optimization

Cui Yang、Xianzhong Chen、Junzhuang Chang、Lihua Zhang、Wei Xu、Wei Shen、You Fan

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Key Laboratory of Carbohydrate Chemistry & Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China

School of Biotechnology, Jiangnan University, Wuxi 214122, China

Supported by the Fundamental Research Funds for the Central UniversitiesSupported by the Fundamental Research Funds for the Central UniversitiesSupported by the Fundamental Research Funds for the Central UniversitiesNatural Science Foundation of Jiangsu ProvinceNatural Science Foundation of Jiangsu ProvinceNational High Technology Research and Development Program of ChinaNational High Technology Research and Development Program of ChinaNational High Technology Research and Development Program of China111 Project

JUSRP51611AJUSRP51504BK20171138863 program2013AA102101-5No.1112-06

2018

中国化学工程学报(英文版)
中国化工学会

中国化学工程学报(英文版)

CSTPCDCSCDSCIEI
影响因子：0.818
ISSN：1004-9541
年,卷(期)：2018.26(12)
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