首页|Vitrification cryopreservation of ligaments based on zwitterionic betaine
Vitrification cryopreservation of ligaments based on zwitterionic betaine
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Ligament cryopreservation enables a prolonged shelf life of allogeneic ligament grafts,which is funda-mentally important to ligament reconstruction.However,conventional cryopreservation techniques fail to eliminate the damage caused by ice crystal growth and the toxicity of cryopreservation agents(CPAs).Here,we report a novel CPA vitrification formulation primarily composed of betaine for ligament cryopreservation.Comprehensive optimization was conducted on the methods for vitrification and rewarming,as well as the loading and unloading conditions,based on the critical cooling rate(CCR),critical warming rate(CWR),and permeation properties of the CPA.Using biomechanical and histological level tests,we demonstrate the superior performance of our method in ligament cryopreservation.After 30 days of vitrification cryopreservation,parameters such as the Young's modulus,tensile stress,denaturation temperature,and glycosaminoglycans content of the ligament remained essentially un-changed.This work pioneers the application of ice-free cryopreservation for ligament and holds great potential for improving the long-term storage of ligament,providing valuable insights for future cryo-preservation technique development.
LigamentBetaineVitrificationCryopreservation
Liming Zhang、Xinmeng Liu、Haoyue Li、Lei Zhang
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Department of Biochemical Engineering,Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(MOE),School of Chemical Engineering and Technology,Tianjin University,Tianjin 300350,China
Department of Biochemical Engineering Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(MOE),School of Chemical Engineering and Technology,Tianjin University,Tianjin 300350,China
Haihe Laboratory of Sustainable Chemical Transformations,Tianjin 300192,China
National Natural Science Foundation of ChinaNational Natural Science Foundation of China
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