Acetic acid-and furfural-based adaptive evolution of Saccharomyces cerevisiae strains for improving stress tolerance and lignocellulosic ethanol production

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外文摘要：Acetic acid and furfural are known as prevalent inhibitors deriving from pretreatment during lignocel-lulosic ethanol production.They negatively impact cell growth,glucose uptake and ethanol biosynthesis of Saccharomyces cerevisiae strains.Development of industrial S.cerevisiae strains with high tolerance towards these inhibitors is thus critical for efficient lignocellulosic ethanol production.In this study,the acetic acid or furfural tolerance of different S.cerevisiae strains could be significantly enhanced after adaptive evolution via serial cultivation for 40 generations under stress conditions.The acetic acid-based adaptive strain SPSC01-TA9 produced 30.5 g·L-1 ethanol with a yield of 0.46 g·g-1 in the presence of 9 g·L-1 acetic acid,while the acetic acid/furfural-based adaptive strain SPSC01-TAF94 produced more ethanol of 36.2 g·L-1 with increased yield up to 0.49 g·g-1 in the presence of both 9 g·L-1 acetic acid and 4 g·L-1 furfural.Significant improvements were also observed during non-detoxified corn stover hy-drolysate culture by SPSC01-TAF94,which achieved ethanol production and yield of 29.1 g·L-1 and 0.49 g g-1,respectively,the growth and fermentation efficiency of acetic acid/furfural-based adaptive strain in hydrolysate was 95％higher than those of wildtype strains,indicating the acetic acid-and furfural-based adaptive evolution strategy could be an effective approach for improving lignocellulosic ethanol production.The adapted strains developed in this study with enhanced tolerance against acetic acid and furfural could be potentially contribute to economically feasible and sustainable lignocellulosic biorefinery.

外文关键词：

Saccharomyces cerevisiaeLignocellulosic ethanol productionAdaptive evolutionAcetic acidFurfural

作者：

Omama Rehman、Youduo Wu、Quan Zhang、Jin Guo、Cuihuan Sun、Huipeng Gao、Yaqing Xu、Rui Xu、Ayesha Shahid、Chuang Xue

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作者单位：

State Key Laboratory of Fine Chemicals,Frontiers Science Centre for Smart Materials Oriented Chemical Engineering,Engineering Research Center of Application and Transformation for Synthetic Biology,School of Bioengineering Dalian University of Technology,Dalian 116024,China

Ningbo Institute of Dalian University of Technology,Ningbo 315016,China

SINOPEC Dalian Research Institute of Petroleum and Petrochemicals Co.,Ltd.,Dalian 116041,China

Microbial Research Institute of Liaoning Province,Chaoyang 122000,China

Yunnan Provincial Rural Energy Engineering Key Laboratory,Kunming 650600,China

US-Pak Center for Advanced Studies in Agriculture and Food Security,University of Agriculture,Faisalabad,Pakistan

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基金：

National Key Research and Development Program of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaMajor Scientific and Technological Projects of SinopecDalian Technology Talents Project for Distinguished Young ScholarsYunnan Provincial Rural Energy Engineering Key LaboratoryNational Natural Science Foundation of Liaoning ProvinceLiaoning Revitalization Talents ProgramFundamental Research Funds for the Central UniversitiesCAS Key Laboratory of Renewable Energy,Guangzhou Institute of Energy Conversion

项目编号：

2021YFC2101303U22A20424223780482021RJ032022KF0032023-MS-110XLYC2202049DUT22LK22E229kf0401

出版年：

2024

DOI：

10.1016/j.cjche.2024.04.002

中国化学工程学报(英文版)

中国化工学会

中国化学工程学报(英文版)

CSTPCDEI

影响因子：0.818

ISSN：1004-9541

年,卷(期)：2024.72(8)