Acetic acid-and furfural-based adaptive evolution of Saccharomyces cerevisiae strains for improving stress tolerance and lignocellulosic ethanol production

Omama Rehman ¹Youduo Wu ²Quan Zhang ³Jin Guo ³Cuihuan Sun ⁴Huipeng Gao ³Yaqing Xu ⁵Rui Xu ⁶Ayesha Shahid ⁷Chuang Xue²

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作者信息

1. State Key Laboratory of Fine Chemicals,Frontiers Science Centre for Smart Materials Oriented Chemical Engineering,Engineering Research Center of Application and Transformation for Synthetic Biology,School of Bioengineering Dalian University of Technology,Dalian 116024,China
2. State Key Laboratory of Fine Chemicals,Frontiers Science Centre for Smart Materials Oriented Chemical Engineering,Engineering Research Center of Application and Transformation for Synthetic Biology,School of Bioengineering Dalian University of Technology,Dalian 116024,China;Ningbo Institute of Dalian University of Technology,Ningbo 315016,China
3. SINOPEC Dalian Research Institute of Petroleum and Petrochemicals Co.,Ltd.,Dalian 116041,China
4. Microbial Research Institute of Liaoning Province,Chaoyang 122000,China
5. Ningbo Institute of Dalian University of Technology,Ningbo 315016,China
6. Yunnan Provincial Rural Energy Engineering Key Laboratory,Kunming 650600,China
7. US-Pak Center for Advanced Studies in Agriculture and Food Security,University of Agriculture,Faisalabad,Pakistan
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Abstract

Acetic acid and furfural are known as prevalent inhibitors deriving from pretreatment during lignocel-lulosic ethanol production.They negatively impact cell growth,glucose uptake and ethanol biosynthesis of Saccharomyces cerevisiae strains.Development of industrial S.cerevisiae strains with high tolerance towards these inhibitors is thus critical for efficient lignocellulosic ethanol production.In this study,the acetic acid or furfural tolerance of different S.cerevisiae strains could be significantly enhanced after adaptive evolution via serial cultivation for 40 generations under stress conditions.The acetic acid-based adaptive strain SPSC01-TA9 produced 30.5 g·L-1 ethanol with a yield of 0.46 g·g-1 in the presence of 9 g·L-1 acetic acid,while the acetic acid/furfural-based adaptive strain SPSC01-TAF94 produced more ethanol of 36.2 g·L-1 with increased yield up to 0.49 g·g-1 in the presence of both 9 g·L-1 acetic acid and 4 g·L-1 furfural.Significant improvements were also observed during non-detoxified corn stover hy-drolysate culture by SPSC01-TAF94,which achieved ethanol production and yield of 29.1 g·L-1 and 0.49 g g-1,respectively,the growth and fermentation efficiency of acetic acid/furfural-based adaptive strain in hydrolysate was 95％higher than those of wildtype strains,indicating the acetic acid-and furfural-based adaptive evolution strategy could be an effective approach for improving lignocellulosic ethanol production.The adapted strains developed in this study with enhanced tolerance against acetic acid and furfural could be potentially contribute to economically feasible and sustainable lignocellulosic biorefinery.

Key words

Saccharomyces cerevisiae/Lignocellulosic ethanol production/Adaptive evolution/Acetic acid/Furfural

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基金项目

National Key Research and Development Program of China(2021YFC2101303)

National Natural Science Foundation of China(U22A20424)

National Natural Science Foundation of China(22378048)

Major Scientific and Technological Projects of Sinopec()

Dalian Technology Talents Project for Distinguished Young Scholars(2021RJ03)

Yunnan Provincial Rural Energy Engineering Key Laboratory(2022KF003)

National Natural Science Foundation of Liaoning Province(2023-MS-110)

Liaoning Revitalization Talents Program(XLYC2202049)

Fundamental Research Funds for the Central Universities(DUT22LK22)

CAS Key Laboratory of Renewable Energy,Guangzhou Institute of Energy Conversion(E229kf0401)

出版年

2024

中国化学工程学报(英文版)

中国化工学会

中国化学工程学报(英文版)

CSTPCDEI

影响因子：0.818

ISSN：1004-9541

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