首页|Plasma membrane-anchored fluorescent tracker based on boron-dipyrromethene

Plasma membrane-anchored fluorescent tracker based on boron-dipyrromethene

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The construction of a stable-membrane tracker has significant implications for the visualization of the membrane in live cells.However,most current plasma trackers are not suitable for tracking plasma membranes for a long time due to their limited retention time.Herein,Mem580-F-Sulfo is designed to target and anchor cell membranes and therefore track cell membranes for a longer time.This tracker is composed of a lipophilic boron-dipyrromethene(BODIPY)derivative and a hydrophilic zwitterion to form an amphiphilic structure,which enables its targeting ability toward cell membranes.Moreover,a reactive ester group is included to bind with proteins through covalent bonds in cell membranes non-specifically,which extends retention time in cell membranes.Mem580-F-Sulfo shows intense bright-ness(94600),with a high molar absorption coefficient of up to about 100000 L·mol-1·cm-1 and a fluorescence quantum yield of up to 0.97.It shows fast cell membrane targeting ability and long retention up to 90 min.In brief,this work has not only developed a tracker with good cell membrane targetability but also provided a new strategy for improving the targeting stability of cell membranes.

Plasma membraneProteinTrackerImagingMicrostructure

Yucong Gong、Xiangli Li、Daqing Ma、Lai Wang、Lin Zhou、Caiwei Lu、Yi Xiao、Xinfu Zhang

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State Key Laboratory of Fine Chemicals,Frontiers Science Center for Smart Materials Oriented Chemical Engineering,Dalian University of Technology,Dalian 116024,China

China Academy of Safety Science and Technology,Beijing 100012,China

National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaFundamental Research Funds for the Central UniversitiesFundamental Research Funds for the Central Universities

222780592217400922078047DUT22-LAB601DUT22LAB608

2024

10.1016/j.cjche.2024.06.002

中国化学工程学报(英文版)
中国化工学会

中国化学工程学报(英文版)

CSTPCDEI
影响因子：0.818
ISSN：1004-9541
年,卷(期)：2024.72(8)