Abstract
The construction of a stable-membrane tracker has significant implications for the visualization of the membrane in live cells.However,most current plasma trackers are not suitable for tracking plasma membranes for a long time due to their limited retention time.Herein,Mem580-F-Sulfo is designed to target and anchor cell membranes and therefore track cell membranes for a longer time.This tracker is composed of a lipophilic boron-dipyrromethene(BODIPY)derivative and a hydrophilic zwitterion to form an amphiphilic structure,which enables its targeting ability toward cell membranes.Moreover,a reactive ester group is included to bind with proteins through covalent bonds in cell membranes non-specifically,which extends retention time in cell membranes.Mem580-F-Sulfo shows intense bright-ness(94600),with a high molar absorption coefficient of up to about 100000 L·mol-1·cm-1 and a fluorescence quantum yield of up to 0.97.It shows fast cell membrane targeting ability and long retention up to 90 min.In brief,this work has not only developed a tracker with good cell membrane targetability but also provided a new strategy for improving the targeting stability of cell membranes.
基金项目
National Natural Science Foundation of China(22278059)
National Natural Science Foundation of China(22174009)
National Natural Science Foundation of China(22078047)
Fundamental Research Funds for the Central Universities(DUT22-LAB601)
Fundamental Research Funds for the Central Universities(DUT22LAB608)
NETL
NSTL
万方数据