Preliminary study on effect on micronucleus rate of peripheral blood erythrocyte of Misgurnus anguillicaudatus induced by copper and zinc
In order to understand the genetic toxicity of copper and zinc to loach(Misgurnus anguillaricaudatus),six copper treatment groups(copper sulfate solution with Cu2+concentration of 0.00,0.02,0.04,0.08,0.10,0.20 mg/L)and seven zinc treatment groups(zinc sulfate solution with Zn2+concentration of 0.0,0.2,0.6,1.1,3.0,5.0,7.0 mg/L)were set respectively on the basis of measuring the half lethal concentration of copper and zinc to loach for 48 hours through acute toxicity tests and obtaining the corresponding safe concentrations.The effect of copper and zinc on the micronucleus rate of peripheral blood erythrocyte of loach was studied by poisoning the loach and collecting blood samples on the 1st,3rd,and 6th day after poisoning.The results showed that after being poisoned with copper sulfate and zinc sulfate,the micronucleus rate of peripheral blood erythrocyte of loaches changed to some extent compared to the control group.Copper sulfate and zinc sulfate can both cause an increase in the micronucleus rate of peripheral blood erythrocyte in loaches to varying degrees,and have significant dose and time effects.Within a certain concentration range,the micronucleus rate is positively correlated with the concentrations of copper sulfate and zinc sulfate;But when the concentration is too high,the micronucleus rate actually decreases with the increase of concentration.
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