Abstract
Critical-sized bone defects,commonly encountered in clinical orthopedic surgery,present a significant challenge.One of the promis-ing solutions is to prepare synthetic bone substitute materials with precise structural control,mechanical compatibility,and enhanced os-teogenic induction performance,nevertheless the successful preparation of such materials remains difficult.In this study,a two-step technique,integrating an extrusion-based printing process with biomimetic mineralization induced by alkaline phosphatase(ALP),was developed.Initially,a pre-cured hydrogel of regenerated silk fibroin(RSF)with a small quantity of hydroxypropyl cellulose(HPC)and ALP was prepared through heat-ing the mixed aqueous solution.This pre-cured hydrogel demonstrated thixotropic property and could be directly extruded into predetermined structures through a 3D-printer.Subsequently,the 3D-printed RSF-based materials with ALP underwent biomimetic in situ mineralization in calci-um glycerophosphate(Ca-GP)mineralizing solution,utilizing the polymer chains of RSF as templates and ALP as a trigger for cleaving phosphate bonds of Ca-GP.The resulting 3D-printed RSF-mineral composites including hydrogel and sponge possessed adjustable compression modulus of megapascal grade and variable hydroxyapatite content,which could be controlled by manipulating the duration of the mineralization process.Moreover,these 3D-printed RSF-mineral composites demonstrated non-cytotoxicity towards rat bone marrow mesenchymal stem cells.There-fore,they may hold great potential for applications involving the replacement of tissues characterized by osteoinductivity and intricate struc-tu res.
基金项目
National Nature Science Foundation of China(21935002)
维普
万方数据