首页|Polyelectrolyte Complexes and Coacervates Formed by De novo-Designed Peptides and Oligonucleotide
Polyelectrolyte Complexes and Coacervates Formed by De novo-Designed Peptides and Oligonucleotide
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The liquid-liquid phase separation of biopolymers in living cells contains multiple interactions and occurs in a dynamic environment.Resolving the regulation mechanism is still a challenge.In this work,we designed a series of peptides(XXLY)6SSSGSS and studied their complexa-tion and coacervation behavior with single-stranded oligonucleotides.The"X"and"Y"are varied to combine known amounts of charged and non-charged amino acids,together with the introduction of secondary structures and pH responsiveness.Results show that the electrostatic in-teraction,which is described as charge density,controls both the strength of complexation and the degree of chain relaxation,and thus deter-mines the growth and size of the coacervates.The hydrophobic interaction is prominent when the charges are neutralized.Interestingly,the sec-ondary structures of peptides exhibit profound effect on the morphology of the phases,such as solid phase to liquid phase transition.Our study gains insight into the phase separation under physiological conditions.It is also helpful to create coacervates with desirable structures and func-tions.
Beijing National Laboratory for Molecular Sciences,Department of Polymer Science and Engineering and the Key Laboratory of Polymer Chemistry and Physics of the Ministry of Education,College of Chemistry and Molecular Engineering,Peking University,Beijing 100871,China
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