Identification of AFLP Markers Linked to Heat-resistant Genes in Hot Pepper
A hybridization between hot pepper heat-resistant inbred line A11 and thermosensitive inbred line B22 was made for mapping heat-resistant gene(s), and the heat resistance of their F2 segregation populations was identified through high temperature stress test. With six Taq I/Ase I primers with high polymorphism screened from 123 primer combinations, AFLP (amplified fragment length polymorphism) analysis was performed on resistant and thermosensitive F 2 plants. A total of about 11 400 distinguishable bands were amplified, of which two were stable difference. Two AFLP markers relevant to heat resistance of hot pepper were obtained preliminarily. The research not only helped to improve the breeding efficiency and level of hot pepper by using marker-assisted selection of heat-resistant genes, but also laid the foundation for the separation and cloning of related heat-resistant functional genes of hot pepper.
NETL
NSTL
万方数据
维普
