首页|Highly sensitive detection of phosphopeptides with superparamagnetic Fe3O4@mZrO2 core-shell microspheres-assisted mass spectrometry

Highly sensitive detection of phosphopeptides with superparamagnetic Fe3O4@mZrO2 core-shell microspheres-assisted mass spectrometry

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Protein phosphorylation is one of the most important post-translational modifications.It is an active research area to study phosphoproteomics for discovery of disease biomarkers and druggable targets.Here we report the development of superparamagnetic Fe3O4@mZrO2 core-shell microspheres with mesoporous structures for highly efficient enrichment of phosphopeptides.We have demonstrated that the mesoporous ZrO2 layer dramatically improves the selective enrichment of phosphopeptides.Our approach allows for in-situ elution and sensitive identification of both mono-phosphorylated and multi-phosphorylated peptides in MALDI-TOF mass spectrometry,with the detection limit of down to the femtomole range.The target phosphopeptides can reliably be enriched for MS analysis from various complex samples including the spiked protein digests and tumor cell lysates.The Fe3O4@mZrO2 core-shell microspheres promise a useful tool for phosphoproteomics by allowing for highly efficient and selective enrichment of the crucial signaling regulators in a low abundance.

Core-shell microspheresMesoporous structureProtein phosphorylationMALDI-TOF mass spectrometryEnrichment technologies

Ruifang Gao、Jin Li、Rui Shi、Yang Zhang、Fuzhou Ouyang、Ting Zhang、Lihua Hu、Guoqiang Xu、Jian Liu

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Institute of Functional Nano & Soft Materials (FUNSOM),Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices,Soochow University,Suzhou 215213,China

Jiangsu Key Laboratory of Neuropsychiatric Diseases and College of Pharmaceutical Sciences Soochow University,Suzhou 215213,China

This work is supported by the National Key Research and Devel-opment Program of ChinaNational Natural Science Foundation of Chinaa project sup-ported by Collaborative Innovation Center of Suzhou Nano Science and Technology111 Project,Joint International Research Lab-oratory of Carbon-Based Functional Materials and DevicesPriority Academic Program Development of Jiangsu Higher Educa-tion Institutions (PAPD)

2017YFE013170021874096

2020

材料科学技术(英文版)
中国金属学会 中国材料研究学会 中国科学院金属研究所

材料科学技术(英文版)

CSTPCDCSCDSCIEI
影响因子：0.657
ISSN：1005-0302
年,卷(期)：2020.59(24)
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