A microfluidic approach for development of hybrid collagen-chitosan extracellular matrix-like membranes for on-chip cell cultures

Erica Rosella ¹Nan Jia ²Diego Mantovani ³Jesse Greener⁴

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作者信息

1. Laboratory, Department of Chemistry, Laval University, Québec, QC, Canada;Laboratory of Biomaterials and Bioengineering, CRC-1, Dept.Min-Met-Materials Eng & CHU de Quebec, Research Center, Laval University, Québec, QC,Canada
2. Laboratory, Department of Chemistry, Laval University, Québec, QC, Canada
3. Laboratory of Biomaterials and Bioengineering, CRC-1, Dept.Min-Met-Materials Eng & CHU de Quebec, Research Center, Laval University, Québec, QC,Canada;CHU de Quebec Research Centre, Laval University, 10 rue de l'Espinay, Québec, QC G1L 3L5, Canada
4. Laboratory, Department of Chemistry, Laval University, Québec, QC, Canada;CHU de Quebec Research Centre, Laval University, 10 rue de l'Espinay, Québec, QC G1L 3L5, Canada
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Abstract

To advance organ-on-a-chip development and other areas befitting from physiologically-relevant biomembranes,a microfluidic platform is presented for synthesis of biomembranes during gelation and investigation into their role as extracellular matrix supports.In this work,high-throughput studies of collagen,chitosan,and collagen-chitosan hybrid biomembranes were carried out to characterize and compare key properties as a function of the applied hydrodynamic conditions during gelation.Specifically,depending on the biopolymer material used,varying flow conditions during biomembrane gelation caused width,uniformity,and swelling ratio to be differently affected and controllable.Finally,cell viability studies of seeded fibroblasts were conducted,thus showing the potential for biological applications.

Key words

Extracellular matrix/Membrane/Natural polymers/Biomaterials/Microfluidics/Fibroblasts

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基金项目

出版年

2021

材料科学技术(英文版)

中国金属学会中国材料研究学会中国科学院金属研究所

材料科学技术(英文版)

CSTPCDCSCDSCI

影响因子：0.657

ISSN：1005-0302

参考文献量30

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