材料科学技术(英文版)2022,Vol.115Issue(20) :52-70.

A novel electrospinning method for self-assembled tree-like fibrous scaffolds:Microenvironment-associated regulation of MSC behavior and bone regeneration

Jeong In Kim Ju Yeon Kim Sung-Ho Kook Jeong-Chae Lee
材料科学技术(英文版)2022,Vol.115Issue(20) :52-70.

A novel electrospinning method for self-assembled tree-like fibrous scaffolds:Microenvironment-associated regulation of MSC behavior and bone regeneration

Jeong In Kim 1Ju Yeon Kim 2Sung-Ho Kook 3Jeong-Chae Lee4
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作者信息

  • 1. Cluster for Craniofacial Development and Regeneration Research,Institute of Oral Biosciences and School of Dentistry,Jeonbuk National University,Jeonju 54896,Republic of Korea
  • 2. Department of Bionanosystem Engineering,Jeonbuk National University,Jeonju 54896,Republic of Korea
  • 3. Department of Bioactive Material Sciences,Research Center of Bioactive Materials,Jeonbuk National University,Jeonju 54896,Republic of Korea
  • 4. Cluster for Craniofacial Development and Regeneration Research,Institute of Oral Biosciences and School of Dentistry,Jeonbuk National University,Jeonju 54896,Republic of Korea;Department of Bioactive Material Sciences,Research Center of Bioactive Materials,Jeonbuk National University,Jeonju 54896,Republic of Korea
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Abstract

Numerous studies highlight advantages of electrospun scaffolds in bone tissue engineering,in which cellular behavior is tightly affected by fiber topographical cues of scaffolds.However,the classic elec-trospinning setup limits a desired presentation of biomimetic fibrous microenvironments that sense mechanosignaling and regulate stem cell behavior.The aims of this study were to fabricate advanced as-spun scaffolds presenting tree-like microfiber/nanonet networks and to evaluate their regulatory poten-tials on behavior of human mesenchymal stem cells(hMSCs)and bone regeneration.Here we developed a novel electrospinning setup that allowed the presentation of patterned Trunk microfibers(TMF)and/or branched nanonet fibers(BNnFs)in biomimetic fibrous scaffolds.As the cellular mechanisms,anisotropic-hierarchical topography of TMF controlled behavior of hMSCs through focal adhesion formation and Yes-associated protein(YAP)induction,whereas BNnF disturbed such mechanosensing responses in the cells.The fiber microenvironment-related expression and nuclear localization of YAP were.also correlated with the potentials of as-spun scaffolds to enhance osteogenic differentiation of the hMSCs and alveolar bone defect healing in an animal model.Collectively,this study provides an advanced approach of the modi-fied electrospinning setup for presentation of biomimetic fibrillar microenvironments in as-spun scaffolds along with their application in stem cell behavior regulation and regenerative tissue engineering.

Key words

Electrospinning/Advanced biomimetic scaffolds/Stem cell behavior/Mechanosensing/Bone tissue engineering

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基金项目

Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Science()

Information and Communica-tions Technology and Future Planning,Republic of Korea(2019R1A2C2084453)

Information and Communica-tions Technology and Future Planning,Republic of Korea(2021R1A2C2006032)

Information and Communica-tions Technology and Future Planning,Republic of Korea(2021R1C1C2011469)

出版年

2022
材料科学技术(英文版)
中国金属学会 中国材料研究学会 中国科学院金属研究所

材料科学技术(英文版)

CSTPCDCSCDSCI
影响因子:0.657
ISSN:1005-0302
被引量1
参考文献量79
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