A novel electrospinning method for self-assembled tree-like fibrous scaffolds:Microenvironment-associated regulation of MSC behavior and bone regeneration

Jeong In Kim ¹Ju Yeon Kim ²Sung-Ho Kook ³Jeong-Chae Lee⁴

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作者信息

1. Cluster for Craniofacial Development and Regeneration Research,Institute of Oral Biosciences and School of Dentistry,Jeonbuk National University,Jeonju 54896,Republic of Korea
2. Department of Bionanosystem Engineering,Jeonbuk National University,Jeonju 54896,Republic of Korea
3. Department of Bioactive Material Sciences,Research Center of Bioactive Materials,Jeonbuk National University,Jeonju 54896,Republic of Korea
4. Cluster for Craniofacial Development and Regeneration Research,Institute of Oral Biosciences and School of Dentistry,Jeonbuk National University,Jeonju 54896,Republic of Korea;Department of Bioactive Material Sciences,Research Center of Bioactive Materials,Jeonbuk National University,Jeonju 54896,Republic of Korea
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Abstract

Numerous studies highlight advantages of electrospun scaffolds in bone tissue engineering,in which cellular behavior is tightly affected by fiber topographical cues of scaffolds.However,the classic elec-trospinning setup limits a desired presentation of biomimetic fibrous microenvironments that sense mechanosignaling and regulate stem cell behavior.The aims of this study were to fabricate advanced as-spun scaffolds presenting tree-like microfiber/nanonet networks and to evaluate their regulatory poten-tials on behavior of human mesenchymal stem cells(hMSCs)and bone regeneration.Here we developed a novel electrospinning setup that allowed the presentation of patterned Trunk microfibers(TMF)and/or branched nanonet fibers(BNnFs)in biomimetic fibrous scaffolds.As the cellular mechanisms,anisotropic-hierarchical topography of TMF controlled behavior of hMSCs through focal adhesion formation and Yes-associated protein(YAP)induction,whereas BNnF disturbed such mechanosensing responses in the cells.The fiber microenvironment-related expression and nuclear localization of YAP were.also correlated with the potentials of as-spun scaffolds to enhance osteogenic differentiation of the hMSCs and alveolar bone defect healing in an animal model.Collectively,this study provides an advanced approach of the modi-fied electrospinning setup for presentation of biomimetic fibrillar microenvironments in as-spun scaffolds along with their application in stem cell behavior regulation and regenerative tissue engineering.

Key words

Electrospinning/Advanced biomimetic scaffolds/Stem cell behavior/Mechanosensing/Bone tissue engineering

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基金项目

Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Science()

Information and Communica-tions Technology and Future Planning,Republic of Korea(2019R1A2C2084453)

Information and Communica-tions Technology and Future Planning,Republic of Korea(2021R1A2C2006032)

Information and Communica-tions Technology and Future Planning,Republic of Korea(2021R1C1C2011469)

出版年

2022

材料科学技术(英文版)

中国金属学会中国材料研究学会中国科学院金属研究所

材料科学技术(英文版)

CSTPCDCSCDSCI

影响因子：0.657

ISSN：1005-0302

被引量1

参考文献量79

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