首页|Engineering Shewanella-reduced graphene oxide aerogel biohybrid to efficiently synthesize Au nanoparticles

Engineering Shewanella-reduced graphene oxide aerogel biohybrid to efficiently synthesize Au nanoparticles

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Biosynthesizing Au nanoparticles(AuNPs)from gold-bearing scraps provides a sustainable method to meet the urgent demand for AuNPs.However,it remains challenging to efficiently biosynthesize AuNPs of which the diameter is less than 10 nm from a trace amount of Au3+concentration at the level of tens ppm.Here,we constructed an exoelectrogenic cell(eCell)-conductive reduced-graphene-oxide aero-gel(rGA)biohybrid by assembling Shewanella sp.S1(SS1)as living biocatalyst and rGA as conductive ad-sorbent,in which Au3+at trace concentrations would be enriched by the adsorption of rGA and reduced to AuNPs through the extracellular electron transfer(EET)of SS1.To regulate the size of the synthe-sized AuNPs to 10 nm,the strain SS1 was engineered to enhance its EET,resulting in strain RS2(pYYD-Ptac-ribADEHC & pHG13-Pbad-omcC in SS1).Strain RS2 was further assembled with rGA to construct the RS2-rGA biohybrid,which could synthesize AuNPs with the size of 7.62±2.82 nm from 60 ppm Au3+so-lution.The eCell-rGA biohybrid integrated Au3+adsorption and reduction,which enabled AuNPs biosyn-thesis from a trace amount of Au3+.Thus,the required Au3+ions concentration was reduced by one or two orders of magnitude compared with conventional methods of AuNPs biosynthesis.Our work devel-oped an AuNPs size regulation technology via engineering eCell's EET with synthetic biology methods,providing a feasible approach to synthesize AuNPs with controllable size from trace level of gold ions.

Au nanoparticlesExoelectrogenic cell(e-Cell)ShewanellaSynthesis biologyExtracellular electron transferReduced graphene oxide aerogel

Baocai Zhang、Shulin Shao、Huan Yu、Qijing Liu、Sicheng Shi、Qingyuan Wu、Zijie Wu、Yiyu Feng、Yifei Wang、Xi Sun、Deguang Wu、Feng Li、Hao Song

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Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education),Tianjin University,Tianjin 300072,China

Collaborative Innovation Center of Chemical Science and Engineering(Tianjin),School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China

Beijing Advanced Innovation Center for Soft Matter Science and Engineering,Beijing University of Chemical Technology,Beijing 100029,China

School of Materials Science and Engineering,Tianjin University,Tianjin 300350,China

College of Biological Engineering,Tianjin Agricultural University,Tianjin 300384,China

Department of Brewing Engineering,Moutai Institute,Luban Ave,Renhuai 564507,China

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国家重点研发计划国家自然科学基金国家自然科学基金国家自然科学基金Young Science and Technology Talents Growth Project of Education Department of Guizhou ProvinceKey Laboratory of Wuliangyeflavor Liquor Solidstate Fermentation,China National Light Industry

2018YFA0901300NSFC 32071411NSFC 32001034NSFC 31701569KY[2018]4452021JJ013

2024

10.1016/j.jmst.2023.04.048

材料科学技术(英文版)
中国金属学会 中国材料研究学会 中国科学院金属研究所

材料科学技术(英文版)

CSTPCD
影响因子：0.657
ISSN：1005-0302
年,卷(期)：2024.168(1)
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