Abstract
Antibacterial Ti-5Cu alloy is a promising substitute material for Ti-made cardiovascular implants,so its surface engineering is crucial to expediting clinical implementation.Given the antibacterial and cardiovas-cular biological benefits of Cu2+and titanium-nitride-oxide(TiNxOy)coatings,a Cu2O/CuO-TiNxOy coating with upregulated Cu2+release was successfully deposited on Ti-5Cu alloy for the first time using oxygen and nitrogen plasma-based surface modification.The superhydrophilic and nanostructured Cu2O/CuO-TiNxOy coating had a dense structure and was well bonded to the substrate,resulting in enhanced cor-rosion resistance,while CuO/Cu2O in the coating released Cu2+faster than Ti2Cu phase in the matrix.More gratifying,the coating demonstrated perfect antibacterial properties(R＞99.9％against S.aureus),owing primarily to direct contact sterilization of Cu2O/CuO.The most encouraging phenomenon was that the coating dramatically accelerated HUVEC adhesion(1.4 times),proliferation(RGR:106％-116％),and particularly migration(RMR:158％-247％)compared with the control Ti.The coating extract also signifi-cantly stimulated in vitro angiogenesis capacity.The rapid endothelialization for Cu2O/CuO-TiNxOy coating was attributed to the surface nanostructure and Cu2+/NO2-release,which upregulated the angiogenesis-related gene expression of HIF-1α,VEGF,and eNOS to increase VEGF secretion and NO production.All of the findings indicated that the Cu2O/CuO-TiNxOy coating could enhance the corrosion resistance,an-tibacterial properties,and endothelialization potential of Ti-Cu alloy,displaying great clinical potential in cardiovascular applications.
基金项目
国家重点研发计划(2022YFE0122800)
Research Program(62602010113)
国家自然科学基金(31971253/C1002)
Beijing Municipal Health Commission(BMHC-2021-6)
Beijing Municipal Health Commission(BMHC-2019-9)
Beijing Municipal Health Commission(PXM 2020_026275_000002)
NETL
NSTL
万方数据