Abstract
Bacterial biofilms,especially those caused by multidrug-resistant bacteria,have emerged as one of the greatest dangers to global public health.The acceleration of antimicrobial resistance to conventional an-tibiotics and the severe lack of new drugs necessitates the development of novel agents for biofilm erad-ication.Photodynamic therapy(PDT)is a promising non-antibiotic method for treating bacterial infec-tions.However,its application in biofilm eradication is hampered by the hypoxic microenvironment of biofilms and the physical protection of extracellular polymeric substances.In this study,we develop a composite nanoplatform with oxygen(O2)self-supplying and heat-sensitizing capabilities to improve the PDT efficacy against biofilms.CaO2/ICG@PDA nanoparticles(CIP NPs)are fabricated by combining calcium peroxide(CaO2)with the photosensitizer indocyanine green(ICG)via electrostatic interactions,followed by coating with polydopamine(PDA).The CIP NPs can gradually generate O2 in response to the acidic microenvironment of the biofilm,thereby alleviating its hypoxic state.Under near-infrared(NIR)irradia-tion,the nanoplatform converts O2 into a significant amount of singlet oxygen(1O2)and heat to eradicate biofilm.The generated heat enhances the release of O2,accelerates the generation of 1O2 in PDT,increases cell membrane permeability,and increases bacterial sensitivity to 1O2.This nanoplatform significantly improves the efficacy of PDT in eradicating biofilm-dwelling bacteria without fostering drug resistance.Experiments on biofilm eradication demonstrate that this nanoplatform can eradicate over 99.9999％of methicillin-resistant Staphylococcus aureus(MRSA)biofilms under 5-min NIR irradiation.Notably,these integrated advantages enable the system to promote the healing of MRSA biofilm-infected wounds with negligible toxicity in vivo,indicating great promise for overcoming the obstacles associated with bacterial biofilm eradication.
基金项目
国家自然科学基金(22175125)
Natural Science Foundation of the Jiangsu Higher Education Institutions of China(21KJA150008)
Key Laboratory of Polymeric Materials Design and Synthesis for Biomedical Function,Soochow University()
江苏省高等学校优势学科建设工程项目()
NETL
NSTL
万方数据