One-pot synthesis of hydroxyapatite hybrid bioinks for digital light processing 3D printing in bone regeneration

Xiaoxiang Ren ¹Jian Wang ²Yan Wu ¹Yuan Zhang ²Jieyuan Zhang ³Long Bai ¹Jinlong Liu ¹Guangfeng Li ⁴Peiran Song ¹Zhongmin Shi ³Jiacan Su⁵

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作者信息

1. Institute of Translational Medicine,Shanghai University,Shanghai 200444,China;Musculoskeletal Organoid Research Center,Shanghai University,Shanghai 200444,China
2. Institute of Translational Medicine,Shanghai University,Shanghai 200444,China;Musculoskeletal Organoid Research Center,Shanghai University,Shanghai 200444,China;School of Medicine,Shanghai University,Shanghai 200444,China
3. National Center for Orthopaedics,Shanghai Sixth People's Hospital,Shanghai 200233,China;Department of Orthopedic Surgery,Shanghai Sixth People's Hospital,Shanghai 200233,China
4. Institute of Translational Medicine,Shanghai University,Shanghai 200444,China;Musculoskeletal Organoid Research Center,Shanghai University,Shanghai 200444,China;School of Medicine,Shanghai University,Shanghai 200444,China;Department of Trauma Orthopedics,Zhongye Hospital,Shanghai 200941,China
5. Institute of Translational Medicine,Shanghai University,Shanghai 200444,China;Musculoskeletal Organoid Research Center,Shanghai University,Shanghai 200444,China;Department of Orthopedic,Xin Hua Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200092,China
折叠

Abstract

Three-dimensional(3D)bioprinting has revolutionized tissue engineering by enabling precise fabrication with bioinks.Among these techniques,digital light processing(DLP)stands out due to its exceptional resolution,speed,and biocompatibility.However,the progress of DLP is hindered by the limited avail-ability of suitable bioinks.Currently,some studies involve simple mixing of different materials,resulting in bioinks that lack uniformity and photopolymerization characteristics.To address this challenge,we present an innovative one-pot synthesis method for bioinks based on methacrylated gelatin/alginate with hydroxyapatite(HAP).This approach offers significant advantages in terms of efficiency and uniformity.The synthesized bioinks demonstrate excellent printability,stability,and notably enhanced mechanical properties,facilitating optimal in vitro compatibility.Additionally,the HAP-hybrid bioinks printed scaf-folds demonstrated impressive bone repair capabilities in vivo compared with pure organic bioinks.In conclusion,the Gel/Alg/HAP bioinks presented herein offer an innovative solution for DLP bioprinting within the field of bone tissue engineering.Their multifaceted advantages help overcome the limitations of restricted bioink choices,pushing forward the boundaries of bioprinting technology and contributing to the progress of regenerative medicine and tissue engineering.

Key words

One-pot strategy/Hybrid bioinks/DIP/Bone regeneration/3D printing

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基金项目

National Natural Science Foundation of China(82202335)

National Natural Science Foundation of China(82230071)

National Natural Science Foundation of China(82172098)

Shanghai Sailing Program(22YF1414000)

出版年

2024

材料科学技术(英文版)

中国金属学会中国材料研究学会中国科学院金属研究所

材料科学技术(英文版)

CSTPCD

影响因子：0.657

ISSN：1005-0302

参考文献量46

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