Abstract
Highly sensitive and stable acetylcholinesterase detection is critical for diagnosing and treating various neurotransmission-related diseases.In this study,a novel colorimetric-fluorescent dual-mode biosen-sor based on highly dispersive trimetal-modified graphite-phase carbon nitride nanocomposites for acetylcholinesterase detection was designed and synthesized by phosphorus doping and a mixed-metal MOF strategy.The specific surface area of trimetal-modified graphite-phase carbon nitride nanocom-posites increased from 15.81 to 96.69 g m-2,and its thermal stability,interfacial charge transfer,and oxidation-reduction capability were enhanced compared with those of graphite-phase carbon ni-tride.First-principles density functional theory calculations and steady-state kinetic analysis are ap-plied to investigate the electronic structures and efficient peroxidase-mimicking properties of trimetal-modified graphite-phase carbon nitride nanocomposites.The oxidation of 3,3',5,5'-tetramethylbenzidin was inhibited by thiocholine,which originates from the decomposition of thiocholine iodide by Acetyl-cholinesterase(AChE),resulting in changes in fluorescence and absorbance intensity.Due to the indepen-dence and complementarity of the signals,a highly precise colorimetric-fluorescent dual-mode biosensor with a linear range for detecting AChE of 4-20 μU mL-1 and detection limits of 0.13 μU mL-1(colori-metric)and 0.04 µU mL-1(fluorescence)was developed.The spiking recovery of AChE in actual samples was 99.0％-100.4％.Therefore,a highly accurate,specific,and stable dual-mode biosensor is available for AChE detection,and this biosensor has the potential for the analysis of other biomarkers.
基金项目
Hubei University of Technology Graduate Research Innovation Project(2022048)
Natural Science Foundation Project of Hubei Province(2022CFB533)
Scientific Research Plan of Education Department of Hubei Province(D20222702)
Natural Science Project of Xiaogan city(XGKJ20210100014)
NETL
NSTL
万方数据