Modeling bacterial adhesion on the nanopatterned surface by varying contact area

Kun Yang ¹Lei Wang ¹Xianrui Zou ²Hongshui Wang ³Chunyong Liang ²Dawei Zhang ⁴Lu-Ning Wang⁵

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作者信息

1. Beijing Advanced Innovation Center for Materials Genome Engineering,State Key Laboratory for Advanced Metals and Materials,School of Materials Science and Engineering,University of Science and Technology Beijing,Beijing 100083,China
2. School of Materials Science and Engineering,Hebei University of Technology,Tianjin 300130,China
3. School of Health Sciences and Biomedical Engineering,Hebei University of Technology Tianjin 300130,China
4. Beijing Advanced Innovation Center for Materials Genome Engineering,State Key Laboratory for Advanced Metals and Materials,School of Materials Science and Engineering,University of Science and Technology Beijing,Beijing 100083,China;Institute for Advanced Materials and Technology,University of Science and Technology Beijing,Beijing 100083,China
5. Beijing Advanced Innovation Center for Materials Genome Engineering,State Key Laboratory for Advanced Metals and Materials,School of Materials Science and Engineering,University of Science and Technology Beijing,Beijing 100083,China;Institute of Materials Intelligent Technology,Liaoning Academy of Materials,Shenyang 110004,China
折叠

Abstract

Bacterial adhesion is a critical process in many fields,such as implant infections,microbiologically influ-enced corrosion and bioelectricity generation in microbial fuel cells.During bacterial adhesion,the con-tact area between the attached bacteria and the patterned surface plays an important role.In this study,different surface topographies and treatments were employed to simulate three circumstances with dif-ferent contact areas.A nanostripe structure with a period of 576.9 nm and a height of 203.5 nm was fabricated on pure titanium by femtosecond laser ablation.Bacteria in liquid attached to the peaks of the nanostripe structure and were stretched on the two adjacent nanostripes.Compared with the polished surface,the contact area between bacteria and the nanostripe surface was reduced to 50％,resulting in a reduction(about 50％)in the coverage rate of attached bacteria.In addition,the nanostripe surface was a hydrophobic surface with a water contact angle(WCA)of 112.1°,and the surface potential of the nanos-tripe surface was higher than that of the polished surface.However,the surface potential and wettability of the nanostripe surface played a minor role in the bacterial adhesion due to the reduced contact area.Upon drying,the attached bacteria on the nanostripe surface sank into the valley region and the contact area was about 40％larger than that on the polished surface.The lateral strength of bacterial adhe-sion on nanostripe surfaces was higher than that on polished surfaces,due to the larger contact area.Upon applying a lateral force of 10.0 nN,the percentage of bacteria remaining on the nanostripe sur-face(31.1％)was higher than that on the polished surface(11.9％).Hence,the bacterial adhesion on the nanopatterned surface was mainly determined by the contact area.The in-depth exploration of the rela-tion between bacterial adhesion on the nanopatterned surface and the contact area enables the rational surface designs of biomaterials to regulate bacterial adhesion.

Key words

Bacterial adhesion/Contact area/Nanopatterned surface/Atomic force microscope

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基金项目

National Natural Science Foundation of China(52071028)

National Natural Science Foundation of China(52003028)

Beijing Nova Program(2022 Beijing Nova Program Cross Cooperation Program)(20220484178)

出版年

2024

材料科学技术(英文版)

中国金属学会中国材料研究学会中国科学院金属研究所

材料科学技术(英文版)

CSTPCDCSCD

影响因子：0.657

ISSN：1005-0302

参考文献量65

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