首页|氮掺杂碳量子点的合成、表征及其在细胞成像中的应用

氮掺杂碳量子点的合成、表征及其在细胞成像中的应用

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以葡萄糖和甘氨酸为混合碳源,在较低温度下经水热法一步合成了氮掺杂的荧光碳量子点(N-CQDs),然后对氮掺杂碳量子点的形貌、结构、组成、光学性质和细胞毒性进行了表征,最后将其应用于细胞成像.实验结果表明,对碳量子点进行氮掺杂能有效提高其荧光量子产率,其荧光增强是由于表面形成了大量强供电子基团,当葡萄糖和甘氨酸的质量比为2∶1时能获得最高为6.57%荧光量子产率.氮掺杂碳量子点还具有水溶性好、粒度均匀、优异的光致发光性质、低的细胞毒性、多波长成像等诸多优点,有望作为荧光探针应用于细胞成像等领域.
Synthesis, Characterization and Cell Imaging Application of Nitrogen-doped Carbon Quantum Dots
Nitrogen-doped carbon quantum dots (N-CQDs) have attracted great interest due to their extraordinary properties,especially their enhanced emission efficiency and thus a facile synthesis of N-CQDs with high emission efficiency is critical for practical applications.Herein,N-CQDs were prepared via one-step hydrothermal treatment using hybrid carbon source (glucose and glycine) at a mild temperature.Morphology,structure,components,and cytotoxicity of the as-prepared N-CQDs were characterized by different instruments.It was found that Nitrogen-doping is an effective method to improve the quantum yield (QY).The enhanced emission efficiency of N-CQDs is due to the formation of strong electron donating groups on their surface.The optimum mass ratio of glucose/glycine is 2∶ 1,and the QY is about 6.57%.The N-CQDs exhibit good water-solubility,well mono-dispersed,good photostability,good salt tolerance and relatively high QY.Furthermore,the N-CQDs also exhibit low toxicity and multicolor imaging properties.All these excellent properties make N-CQDs being a useful candidate of probes for cell imaging.

Carbon quantum dotsNitrogen-dopedCell imaging

李晓峰、周明、龚爱华、张严、杨青峰

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江苏大学光子制造与技术中心,江苏镇江212013

清华大学摩擦学国家重点实验室,北京100084

江苏大学基础医学与医学技术学院,江苏镇江212013

碳量子点 氮掺杂 细胞成像

国家973计划资助项目国家自然科学基金资助项目清华大学摩擦学重点实验室重点项目

2011CB01300450975128SKLT10A02

2015

材料科学与工程学报
浙江大学

材料科学与工程学报

CSTPCDCSCD北大核心
影响因子:0.765
ISSN:1673-2812
年,卷(期):2015.33(1)
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