Synthesis, Characterization and Cell Imaging Application of Nitrogen-doped Carbon Quantum Dots
Nitrogen-doped carbon quantum dots (N-CQDs) have attracted great interest due to their extraordinary properties,especially their enhanced emission efficiency and thus a facile synthesis of N-CQDs with high emission efficiency is critical for practical applications.Herein,N-CQDs were prepared via one-step hydrothermal treatment using hybrid carbon source (glucose and glycine) at a mild temperature.Morphology,structure,components,and cytotoxicity of the as-prepared N-CQDs were characterized by different instruments.It was found that Nitrogen-doping is an effective method to improve the quantum yield (QY).The enhanced emission efficiency of N-CQDs is due to the formation of strong electron donating groups on their surface.The optimum mass ratio of glucose/glycine is 2∶ 1,and the QY is about 6.57%.The N-CQDs exhibit good water-solubility,well mono-dispersed,good photostability,good salt tolerance and relatively high QY.Furthermore,the N-CQDs also exhibit low toxicity and multicolor imaging properties.All these excellent properties make N-CQDs being a useful candidate of probes for cell imaging.