慢性乙型肝炎患者肝组织中ICAM-1、NF-κB表达与肝脏炎症活动度及纤维化程度的相关性分析
Correlation analysis of ICAM-1 and NF-κB expression with hepatic inflammatory activity and degree of fibrosis in liver tissues of chronic hepatitis B patients
李涛 1李日林 2谢宗恕 2杨秀珍 3佘东明 3刘志娟 3袁淑芳3
作者信息
- 1. 桂林医学院,广西桂林 541004
- 2. 广西医科大学,南宁 530021
- 3. 柳州市人民医院感染科,广西柳州 545006
- 折叠
摘要
目的 检测慢性乙型肝炎患者肝组织中细胞间黏附分子-1(ICAM-1)和核因子(NF)-κB表达,分析其与肝脏炎症活动度和纤维化程度的关系.方法 选取66例慢性乙型肝炎患者和10例非肝病对照者的肝活检标本,免疫组织化学和原位杂交法检测不同肝组织中ICAM-1、NF-κB蛋白表达情况.结果 慢性乙型肝炎患者肝组织中ICAM-1、NF-κB蛋白阳性表达率高于正常肝组织,差异有统计学意义(P＜0.05).慢性乙型肝炎患者肝组织中ICAM-1、NF-κB蛋白表达与炎症活动度和纤维化程度呈正相关(r=0.493、0.496,P＜0.01;r=0.580、0.519,P＜0.01).结论 ICAM-1、NF-κB在慢性乙型肝炎患者中高表达,其有助于判断肝脏炎症活动度和纤维化程度.
Abstract
Objective To detect the expressions of intercellular adhesion molecule-1(ICAM-1)and nu-clear factor(NF)-κB in hepatic tissues of the patients with chronic hepatitis B,and to analyze their correlation with the hepatic inflammatory activity and fibrosis degree.Methods The liver biopsy specimens from 66 pa-tients with hepatitis B and 10 non-hepatopathic controls were selected,and immunohistochemistry and in situ hybridization were used to detect ICAM-1 and NF-κB expression levels in different liver tissues.Results The positive rate of ICAM-1 and NF-κB expression in liver tissues of the patients with chronic hepatitis B was higher than that in normal liver tissues,and the difference was statistically significant(P＜0.05).The expres-sion of ICAM-1 and NF-κB in the patients with hepatitis B was positively correlated with the inflammatory ac-tivity and fibrosis degree(r=0.493,0.496,P＜0.01;r=0.580,0.519,P＜0.01).Conclusion ICAM-1 and NF-κB in the patients with chronic hepatitis B are highly expressed,which is useful in judging the hepatic in-flammatory activity and fibrosis degree.
关键词
细胞间黏附分子-1/核因子κB/慢性乙型肝炎Key words
intercellular adhesion molecule-1/nuclear factor kappa-B/chronic hepatitis B引用本文复制引用
基金项目
柳州市科技局项目(2020NBAB0821)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据